The prognostic value of hTERT expression levels in advanced-stage colorectal cancer patients: a comparison between tissue and serum expression
The prognostic value of hTERT expression levels in advanced-stage colorectal cancer patients: a comparison between tissue and serum expression
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DOI：
10.1007/s12094-011-0673-2
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发表时间：
2011-06-01
影响因子：
3.4
通讯作者：
Camps, Carlos
中科院分区：
文献类型：
--
作者：
Jose Safont, Maria;Gil, Mireia;Camps, Carlos
目的端粒是位于染色体末端的高度重复的非编码DNA区域，其主要功能是维持这些末端的结构稳定性。在90%的人类肿瘤中，端粒长度通过端粒酶逆转录酶的表达和激活来维持。各种研究表明，肿瘤组织中端粒酶活性增加，这表明其可能的预后价值。为探讨端粒酶催化蛋白（hTERT）表达水平对结直肠癌（CRC）患者的预后价值，采用免疫组化方法检测肿瘤组织中端粒酶表达与癌旁正常粘膜端粒酶表达的比值，并与血清中hTERT表达水平进行比较。作为本研究的第二个目的，我们（1）分析了端粒酶在血清和肿瘤组织中的表达之间的相关性，（2）分析了端粒酶表达与不同临床参数之间的关系。在基于肿瘤组织和健康组织之间的端粒酶表达比率分类的患者组之间，未观察到无病生存期（DFS）或总生存时间（OST）的显著差异。血清端粒酶水平与组织端粒酶水平的相关系数为0.32。我们的研究端粒酶水平在血清中和不同的临床变量，如肿瘤大小，神经节的影响，术前癌胚抗原水平和阶段之间的关系，揭示了较高的端粒酶表达水平在IV期CRC患者。肿瘤组织中端粒酶的表达和临床参数analysed.Conclusions之间没有显着的关联，在我们的研究中获得的结果不允许我们提出，端粒酶的表达水平被用作结直肠癌的预后因素。因此，我们不能将血清中端粒酶的表达作为肿瘤组织中端粒酶表达的替代标志物。
Aim Telomeres are regions of highly repetitive, non-coding DNA located at the termini of chromosomes whose principal function is to maintain the structural stability of these ends. In 90% of human tumours, telomere length is maintained by the expression and activation of telomerase reverse transcriptase. Various studies have demonstrated an increase in telomerase activity in tumour tissue, which suggests its possible prognostic value. The main objective of our study was to study the prognostic value of the expression level of telomerase catalytic component (hTERT) in patients with colorectal cancer (CRC).Methods We analysed the prognostic value of the ratio of telomerase expression in tumour tissue to telomerase expression in the adjacent healthy mucosa and the prognostic value of the expression level of hTERT in the serum of patients diagnosed with CRC. As secondary objectives of the study, we (1) analysed the correlation between telomerase expression in the serum and that in the tumour tissue and (2) analysed the relationship between telomerase expression and different clinical parameters.Results Peripheral blood and tissue samples taken from 48 patients with CRC were analysed. No significant differences were observed in disease-free survival (DFS) or overall survival time (OST) between the groups of patients categorised based on the ratio of telomerase expression between tumour tissue and healthy tissue. The correlation index (Pearson's coefficient) between telomerase levels in the serum and those in tissue was 0.32. Our study of the relationship between telomerase levels in the serum and different clinical variables, such as tumour size, ganglion affectation, preoperative carcinoembryonic antigen levels and stage, revealed a higher telomerase expression level in patients with stage IV CRC. There was no significant association between telomerase expression in tumour tissue and the clinical parameters analysed.Conclusions The results obtained in our study do not allow us to propose that the level of telomerase expression be used as a prognostic factor in colorectal cancer. Thus, we cannot consider telomerase expression in the serum as a surrogate marker of its expression in tumour tissue.