Overexpression of the ethylene-responsive factor gene BrERF4 from Brassica rapa increases tolerance to salt and drought in Arabidopsis plants
Overexpression of the ethylene-responsive factor gene BrERF4 from Brassica rapa increases tolerance to salt and drought in Arabidopsis plants
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DOI：
10.1007/s10059-010-0114-z
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发表时间：
2010-08
影响因子：
3.8
通讯作者：
Yean Joo Seo;Jong-Beum Park;Yeon-Jeong Cho;Choonkyun Jung;H. Seo;Soon-Ki Park;B. Nahm;J. Song
中科院分区：
文献类型：
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作者：
Yean Joo Seo;Jong-Beum Park;Yeon-Jeong Cho;Choonkyun Jung;H. Seo;Soon-Ki Park;B. Nahm;J. Song
乙烯应答因子（Ethylene-responsive factors，ERFs）是植物对生物或非生物胁迫的反应中的一类转录因子，属于AP 2/ERF转录因子家族的一个亚类。在这里，我们报告了一个ERF基因从白菜（BrERF 4）的过表达导致提高耐盐性和干旱胁迫拟南芥。对转基因植株的生长发育也有显著影响。我们检测到，盐诱导的转录抑制基因AtERF 4和一些Ser/Thr蛋白磷酸酶2C基因ABI 1，ABI 2和AtPP 2CA的表达在BrERF 4过表达的拟南芥中被抑制。此外，BrERF 4在B中被乙烯或茉莉酸甲酯诱导，而不被脱落酸或NaCl诱导。拉帕。这些结果表明，BrERF 4通过不同的信号通路网络激活，以响应盐度和干旱。
Ethylene-responsive factors (ERFs), within a subgroup of the AP2/ERF transcription factor family, are involved in diverse plant reactions to biotic or abiotic stresses. Here, we report that overexpression of an ERF gene fromBrassica rapassp.pekinensis(BrERF4) led to improved tolerance to salt and drought stresses in Arabidopsis. It also significantly affected the growth and development of transgenic plants. We detected that salt-induced expressions of a transcriptional repressor gene,AtERF4, and some Ser/Thr protein phosphatase2C genes,ABI1, ABI2andAtPP2CA, were suppressed inBrERF4-overexpressing Arabidopsis plants. Furthermore,BrERF4was induced by treatment with ethylene or methyljasmonate, but not by abscisic acid or NaCl inB. rapa. These results suggest that BrERF4 is activated through a network of different signaling pathways in response to salinity and drought.