Quinclorac Resistance in Echinochloa crus-galli from China
Quinclorac Resistance in Echinochloa crus-galli from China
复制标题
中国稗草的二氯喹啉酸抗性
DOI：
10.1016/j.rsci.2019.08.004
复制
发表时间：
2019-09-01
期刊：
影响因子：
4.8
通讯作者：
Powles, Stephen B.
中科院分区：
文献类型：
--
作者：
Peng Qiong;Han Heping;Powles, Stephen B.
稗草是我国水稻田的主要杂草，二氯喹啉酸是防治稗草的常用药剂。对二氯喹啉酸的过度依赖导致了E.我的老天爷两个抗性（R）E.来自中国湖南的稗草种群被证实对二氯喹啉酸的抗性比敏感种群（S）高至少78倍。在R和S植物之间检测到C-14标记的二氯喹啉酸的叶片吸收没有差异。然而，较高水平的C-14易位和较低水平的二氯喹啉酸代谢被发现在R植物。β-氰丙氨酸合酶（β-CAS）基因的基础和诱导表达水平和β-CAS活性在R和S植物之间没有显着差异。二氯喹啉酸处理对1-氨基环丙烷-1-羧酸氧化酶（ACO 1）基因的表达有明显的诱导作用，但对抗性植株的诱导作用不明显。两株抗性E. crus-galli种群不太可能与叶片吸收、二氯喹啉酸的转运或代谢有关，也不可能与通过β-CAS进行的氰化物解毒有关。因此，基于靶点的二氯喹啉酸信号接收、传递和乙烯合成途径的调控是今后研究的重点。
Echinochloa crus-galli is a major weed in rice fields in China, and quinclorac has been long used for its control. Over-reliance of quinclorac has resulted in quinclorac resistance in E. crus-galli. Two resistant (R) E. crus-galli populations from Hunan, China were confirmed to be at least 78-fold more resistant to quinclorac than the susceptible (S) population. No difference in foliar uptake of C-14-labelled quinclorac was detected between the R and S plants. However, a higher level of C-14 translocation and a lower level of quinclorac metabolism were found in the R plants. Basal and induced expression levels of beta-cyanoalanine synthase (beta-CAS) gene and beta-CAS activity were not significantly different between the R and S plants. However, the induction expression of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1) gene by quinclorac treatment was evident in the S plants but not in the R plants. Quinclorac resistance in the two resistant E. crus-galli populations was not likely to be related to foliar uptake, translocation or metabolism of quinclorac, nor to cyanide detoxification via beta-CAS. Thus, target-site based quinclorac signal reception and transduction and regulation of the ethylene synthesis pathway should be the focus for further research.