Rhizobium ipomoeae sp. nov., isolated from a water convolvulus field.
Rhizobium ipomoeae sp. nov., isolated from a water convolvulus field.
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DOI：
10.1099/ijsem.0.000875
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发表时间：
2016-04
影响因子：
2.8
通讯作者：
Shih-Yi Sheu;Zih-Han Chen;C. Young;Wen‐ming chen
中科院分区：
文献类型：
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作者：
Shih-Yi Sheu;Zih-Han Chen;C. Young;Wen‐ming chen
从台湾水旋花田的水样中分离出一种名为 shin9-1T 的细菌菌株，并使用多相分类学方法对其进行了表征。 shin9-1T菌株的细胞是需氧的、革兰氏染色阴性的、杆状的并且被厚囊包围并形成奶油色的菌落。生长发生在 10-45 °C（最佳，30 °C）、0-3.0% NaCl（最佳，0.5%）和 pH 7.0-9.0（最佳，pH 7.0）的条件下。菌株shin9-1T在豆科植物Macroptilium atropurpureum上未形成根瘤，并且PCR未检测到结瘤基因nodA、nodC和固氮酶还原酶基因nifH。基于16S rRNA和三个管家基因序列（recA、atpD和rpoB）的系统发育分析表明，菌株shin9-1T属于根瘤菌属。菌株shin9-1T与大田根瘤菌L61T的16S rRNA基因序列相似性最高(97.6%)。菌株shin9-1T的主要脂肪酸是C18:1ω7c。极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、二磷脂酰甘油、磷脂酰单甲基乙醇胺和几种未表征的脂质组成。 DNA G+C含量为58.3mol%。菌株shin9-1T与根瘤菌属已知物种的DNA-DNA相关性小于70%。新菌株的表型特征也不同于根瘤菌属最密切相关的物种。根据系统发育推断和表型数据，菌株shin9-1T应被归类为新物种的代表，其名称为Rhizobium ipomoeae sp。十一月被提议。模式菌株是 shin9-1T (=LMG 27163T=KCTC 32148T)。
A bacterial strain, designated shin9-1T, was isolated from a water sample taken from a water convolvulus field in Taiwan and characterized using a polyphasic taxonomical approach. Cells of strain shin9-1T were aerobic, Gram-stain-negative, rod-shaped and surrounded by a thick capsule and formed cream-coloured colonies. Growth occurred at 10-45 °C (optimum, 30 °C), with 0-3.0% NaCl (optimum, 0.5%) and at pH 7.0-9.0 (optimum, pH 7.0). Strain shin9-1T did not form nodules on a legume plant, Macroptilium atropurpureum, and the nodulation genes nodA, nodC and the nitrogenase reductase gene nifH were not detected by PCR. Phylogenetic analyses based on 16S rRNA and three housekeeping gene sequences (recA, atpD and rpoB) showed that strain shin9-1T belonged to the genus Rhizobium. Strain shin9-1T had the highest level of 16S rRNA gene sequence similarity with respect to Rhizobium daejeonense L61T (97.6 %). The major fatty acid of strain shin9-1T was C18:1ω7c. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine and several uncharacterized lipids. The DNA G+C content was 58.3 mol%. The DNA-DNA relatedness of strain shin9-1T with respect to recognized species of the genus Rhizobium was less than 70%. Phenotypic characteristics of the novel strain also differed from those of the most closely related species of the genus Rhizobium. On the basis of the phylogenetic inference and phenotypic data, strain shin9-1T should be classified as a representative of a novel species, for which the name Rhizobium ipomoeae sp. nov. is proposed. The type strain is shin9-1T (=LMG 27163T=KCTC 32148T).