FUNCTIONAL-ROLE OF VLA-1 (CD49A) IN ADHESION, CATION-DEPENDENT SPREADING, AND ACTIVATION OF CULTURED HUMAN T-LYMPHOCYTES
FUNCTIONAL-ROLE OF VLA-1 (CD49A) IN ADHESION, CATION-DEPENDENT SPREADING, AND ACTIVATION OF CULTURED HUMAN T-LYMPHOCYTES
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DOI：
10.1006/cimm.1994.1187
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发表时间：
1994-07-01
影响因子：
4.3
通讯作者：
WARE, R
中科院分区：
文献类型：
--
作者：
BANK, I;BOOK, M;WARE, R
研究了VLA-1（CD49a，α 1 β 1整合素）在体内活化的人T淋巴细胞上的功能。在一组25个长期培养的IL-2依赖性T细胞系中，tau δ和CD8（+）α β细胞表达的α 1 β 1水平显著高于CD4（+）α β细胞。当VLA-1-高tau δ或CD 8（+）细胞粘附于涂有胶原IV、胶原I或纤连蛋白的塑料威尔斯孔时，针对VLA-1的单克隆抗体1B3.1仅抑制对胶原IV的粘附。在Mg2+存在下，在胶原IV上分层的tau δ和CD8（+）VLA-1-高T细胞也伸展伸长的细胞质延伸，其被moAb 1B3.1消除。相反，纤连蛋白上的扩散或自发的非ECM相关扩散未被抑制。表面VLA-1分子与塑料结合的单克隆抗体1B3.1的交联选择性地诱导6个VLA-1（+）克隆中的两个上的IL-2R表达，这两个克隆都表达tau δ TCR。因此，CD49a是VLA-1-高tau δ和CD8（+）α β细胞中的特异性胶原IV受体，并且可以将信号传递给这些淋巴细胞以扩散和表达IL-2R。(C)1994年出版社出版。
The functions of VLA-1 (CD49a, alpha 1 beta 1 integrin), a potential ECM protein receptor on activated human T lymphocytes in vivo, were investigated. Within a panel of 25 long-term cultured IL-2-dependent T cell lines, tau delta and CD8(+)alpha beta cells expressed significantly higher levels of alpha 1 beta 1 than CD4(+)alpha beta cells. While VLA-1-high tau delta or CD8(+) cells adhered to plastic wells coated with collagen IV, collagen I, or fibronectin, moAb 1B3.1 to VLA-1 only inhibited the adherence to collagen IV. tau delta and CD8(+) VLA-1-high T cells layered upon collagen IV in the presence of Mg2+ also spread elongated cytoplasmic extensions, which were abrogated by moAb 1B3.1. In contrast, spreading on fibronectin or spontaneous non-ECM-related spreading were not inhibited. Crosslinking of surface VLA-1 molecules with plastic-bound moAb 1B3.1 selectively induced expression of IL-2R on two of six VLA-1(+) clones, both of which expressed tau delta TCR. Thus, CD49a is a specific collagen IV receptor in VLA-1-high tau delta and CD8(+)alpha beta cells and can transmit signals to these lymphocytes to spread and express IL-2R. (C) 1994 Academic Press, Inc.