Curcumin attenuates inflammatory responses by suppressing TLR4-mediated NF-κB signaling pathway in lipopolysaccharide-induced mastitis in mice
Curcumin attenuates inflammatory responses by suppressing TLR4-mediated NF-κB signaling pathway in lipopolysaccharide-induced mastitis in mice
复制标题
DOI：
10.1016/j.intimp.2014.01.024
复制
发表时间：
2014-05-01
影响因子：
5.6
通讯作者：
Zhang, Naisheng
中科院分区：
文献类型：
--
作者：
Fu, Yunhe;Gao, Ruifeng;Zhang, Naisheng
姜黄素是香料姜黄的主要成分，据报道具有有效的抗炎特性。然而，姜黄素对脂多糖（LPS）诱导的小鼠乳腺炎的影响尚未被研究。本研究的目的是探讨姜黄素是否可以改善脂多糖诱导的小鼠乳腺炎的炎症反应，并阐明其可能的机制。通过乳腺导管注射LPS诱导小鼠乳腺炎模型。 LPS处理前1小时和LPS处理后12小时施用姜黄素。结果表明，姜黄素以剂量依赖性方式减弱炎症细胞的浸润、髓过氧化物酶（MPO）的活性以及肿瘤坏死因子-α（TNF-α）、白细胞介素6（IL-6）和白细胞介素1β（IL-1β）的表达。此外，蛋白质印迹结果表明姜黄素抑制 I kappa B-alpha 的磷酸化。 NF-κB p65 和 TLR4 的表达。这些结果表明姜黄素对小鼠乳腺炎具有保护作用，姜黄素对LPS诱导的小鼠乳腺炎的抗炎机制可能与其抑制TLR4介导的NF-κB信号通路有关。姜黄素可能是一种潜在的乳腺炎治疗剂。 2014 Elsevier B.V. 保留所有权利。
Curcumin, the main constituent of the spice turmeric, has been reported to have potent anti-inflammatory properties. However, the effect of curcumin on lipopolysaccharide (LPS)-induced mice mastitis has not been investigated. The aim of this study was to investigate whether curcumin could ameliorate the inflammation response in LPS-induced mice mastitis and to clarify the possible mechanism. The mouse model of mastitis was induced by injection of LPS through the duct of the mammary gland. Curcumin was applied 1 h before and 12 h after LPS treatment. The results showed that curcumin attenuated the infiltration of inflammatory cells, the activity of myeloperoxidase (MPO), and the expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) in a dose-dependent manner. Additionally, Western blotting results showed that curcumin inhibited the phosphorylation of I kappa B-alpha. and NF-kappa B p65 and the expression of TLR4. These results indicated that curcumin has protective effect on mice mastitis and the anti-inflammatory mechanism of curcumin on LPS-induced mastitis in mice may be due to its ability to inhibit TLR4-mediated NF-kappa B signaling pathways. Curcumin may be a potential therapeutic agent against mastitis. 2014 Elsevier B.V. All rights reserved.