Characterization of Klebsiella pneumoniae Extracellular Polysaccharides.
Characterization of Klebsiella pneumoniae Extracellular Polysaccharides.
复制标题
肺炎克雷伯菌胞外多糖的表征。
DOI：
10.1002/cpz1.937
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Mike,LauraA
中科院分区：
文献类型：
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作者：
Khadka,Saroj;Ring,BrookeE;Pariseau,DrewA;Mike,LauraA
肺炎克雷伯菌是一种具有临床意义的革兰氏阴性病原体，其细胞外多糖的产生是关键的毒力因子。胞外多糖如荚膜及其粘液粘度在克氏杆菌中起着重要作用。肺炎感染。在本文中，我们解释了用于表征 K 细胞外多糖的几种标准方案。肺炎杆菌。其中一些协议专门针对 K 进行了修改。肺炎克雷伯菌并描述了纯化和定量肺炎克雷伯菌胞外多糖的方法。肺炎杆菌。我们还提出了一个标准化协议来量化 K。肺炎粘液粘度是克雷伯菌的独特特征。肺炎细胞外多糖。这些协议将有助于创建 inK 使用的标准协议的统一性。肺炎细胞外多糖研究。 © 2023 作者。 Wiley periodicals LLC 出版的最新方案。 基本方案 1：细胞外多糖分离和纯化基本方案 2：细胞外多糖的大规模分离和纯化基本方案 3：胞外多糖的糖醛酸定量基本方案 4：通过 SDS-PAGE 进行胞外多糖可视化基本方案 5：克雷伯氏菌 通过沉降阻力测定法测量肺炎粘液粘度替代方案 5：基于 96 孔板的肺炎克雷伯氏菌沉降阻力测定支持方案 5：测定板到比色皿转换因子
Klebsiella pneumoniaeis a clinically significant, Gram‐negative pathogen in which the production of extracellular polysaccharides is a key virulence factor. Extracellular polysaccharides such as the capsule and its mucoviscosity play a significant role inK. pneumoniaeinfection. In this article, we explain several standard protocols used to characterize the extracellular polysaccharides ofK. pneumoniae. Several of these protocols are adapted specifically forK. pneumoniaeand describe methods to purify and quantify the extracellular polysaccharide ofK. pneumoniae. We also present a standardized protocol to quantifyK. pneumoniaemucoviscosity, a unique feature ofK. pneumoniaeextracellular polysaccharide. These protocols will help create uniformity in standard protocols used inK. pneumoniaeextracellular polysaccharide studies. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Extracellular polysaccharide isolation and purificationBasic Protocol 2: Large‐scale isolation and purification of extracellular polysaccharideBasic Protocol 3: Uronic acid quantification of extracellular polysaccharideBasic Protocol 4: Extracellular polysaccharide visualization by SDS‐PAGEBasic Protocol 5:Klebsiella pneumoniaemucoviscosity measurement by sedimentation resistance assayAlternate Protocol 5: 96‐well plate‐basedKlebsiella pneumoniaesedimentation resistance assaySupport Protocol 5: Determination of plate to cuvette conversion factor