SPECIFIC HIGH-AFFINITY RECEPTORS FOR NEUROTROPHIN-3 ON SYMPATHETIC NEURONS
SPECIFIC HIGH-AFFINITY RECEPTORS FOR NEUROTROPHIN-3 ON SYMPATHETIC NEURONS
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DOI：
10.1523/jneurosci.13-06-02610.1993
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发表时间：
1993-06-01
影响因子：
5.3
通讯作者：
BARDE, YA
中科院分区：
文献类型：
--
作者：
DECHANT, G;RODRIGUEZTEBAR, A;BARDE, YA
当使用的浓度只允许与其高亲和力受体的相互作用，神经营养素-3（NT-3）促进从胚胎第8天（E8）小鸡分离的感觉神经元的存活，但不E11交感神经元的存活。这些交感神经元（可以通过添加NGF来拯救）显示出NT-3的高亲和力受体（K（d）为1.6 × 10（-11）M），使用平衡结合或动力学标准无法将其与感觉神经元上的高亲和力NT-3受体区分开来。这代表了胚胎神经元的第一个例子，不能通过在体外添加神经营养因子，尽管存在相应的神经营养因子高亲和力受体的拯救。在升高的浓度下，超过其高亲和力受体的饱和度，NT-3支持一些E11交感神经元的存活，这种作用可能是由高亲和力NGF受体介导的。使用E7交感神经元，最初接种的约40%的细胞可以通过添加低浓度的NT-3（但不是NGF）在体外被拯救，并产生丰富的神经突。提示NT-3可能在某些交感神经元的早期发育中起作用。
When used at concentrations allowing interactions only with its high-affinity receptors, neurotrophin-3 (NT-3) promotes the survival of sensory neurons isolated from embryonic day 8 (E8) chicks, but not the survival of E11 sympathetic neurons. These sympathetic neurons (which can be rescued by the addition of NGF) display high-affinity receptors for NT-3 (K(d) of 1.6 x 10(-11) M) that cannot be distinguished from the high-affinity NT-3 receptors on sensory neurons using equilibrium binding or kinetic criteria. This represents the first example of embryonic neurons that cannot be rescued by the in vitro addition of a neurotrophin in spite of the presence of corresponding neurotrophin high-affinity receptors. At elevated concentrations, beyond the saturation of its high-affinity receptors, NT-3 supports the survival of some E11 sympathetic neurons, an effect that might be mediated by the high-affinity NGF receptor. Using E7 sympathetic neurons, about 40% of the cells initially plated can be rescued in vitro by the addition of low concentrations of NT-3 (but not of NGF) and produce profuse neurites. This indicates that NT-3 may play a role in the early development of some sympathetic neurons.