Promotion of bioremediation performance in constructed wetland microcosms for acid mine drainage treatment by using organic substrates and supplementing domestic wastewater and plant litter broth
Promotion of bioremediation performance in constructed wetland microcosms for acid mine drainage treatment by using organic substrates and supplementing domestic wastewater and plant litter broth
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使用有机基质并补充生活废水和植物枯落物培养液，促进酸性矿山排水处理的人工湿地微观世界的生物修复性能
DOI：
10.1016/j.jhazmat.2020.124125
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发表时间：
2021-02-15
影响因子：
13.6
通讯作者：
Chang, Junjun
中科院分区：
文献类型：
--
作者：
Chen, Jinquan;Li, Xuan;Chang, Junjun
本研究以核桃壳（WS）基质为基质，建立了碎石基地下流人工湿地（CWs），用于处理合成酸性矿山废水（AMD），并添加了人工生活废水（DW）和植物凋落物肉汤（PLB）来提高湿地的处理性能。在没有外部碳源的情况下，CW介质对金属（除了Fe和Cr）的吸附迅速达到饱和，而DW和PLB的加入刺激了硫酸盐还原活性，并通过形成氢氧化物和硫化物沉淀以及伴随的共沉淀实现了高效的生物源金属去除。与对照系统相比，WS改良的CWs不仅在固存更多的金属和快速建立有利的生物源金属减排环境方面表现明显更好，而且在支持植物和功能微生物更好的生长方面也表现得更好。外部有机碳输入极大地塑造了CWs中的细菌群落组成，参与AMD生物处理的核心功能种群的比例大幅增加。以Desulfobulbus和desulfatirhadium为主的纤维素单胞菌（Cellulomonas）、丙酸菌（propioniclava）和硫酸盐还原菌（sulfate-reducing bacteria， SRB）的协同作用是海洋环境中AMD修复的主要生物机制。添加DW和PLB的纤维素废物改性化粪池是一种很有前途的AMD修复生态技术。
Gravel-based subsurface-flow constructed wetlands (CWs) amended with a walnut shell (WS) substrate were established to treat synthetic acid mine drainage (AMD) in this study, and artificial domestic wastewater (DW) and plant litter broth (PLB) were supplemented to enhance the performance. The CW media rapidly reached adsorption saturation with respect to metals (except Fe and Cr) without an external carbon source, while the addition of DW and PLB stimulated sulfate reduction activity and achieved efficient biogenic metal removal, primarily by the formation of hydroxide and sulfide precipitates and concomitant co-precipitation. The WS amended CWs performed notably better than the control systems, not only in sequestering more metals and rapidly establishing favourable environments for biogenic metal abatement but also in supporting better growth of plants and functional microbes. The external organic carbon input greatly shaped the bacterial community compositions in the CWs, with substantial increases in the proportions of core functional populations involved in AMD biotreatment. Cooperation among Cellulomonas, Propioniciclava and sulfate-reducing bacteria (SRB), dominated by Desulfobulbus and Desulfatirhabdium, was the primary biogenic mechanism of AMD remediation in the CWs. Cellulosic waste-amended CWs with DW and PLB addition offer a promising eco-technology for AMD remediation.