DIFFERENTIAL SYNTHESIS INVITRO OF BARLEY ALEURONE AND STARCHY ENDOSPERM PROTEINS
DIFFERENTIAL SYNTHESIS INVITRO OF BARLEY ALEURONE AND STARCHY ENDOSPERM PROTEINS
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DOI：
10.1104/pp.81.2.630
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发表时间：
1986-06-01
期刊：
影响因子：
7.4
通讯作者：
MUNCK, L
中科院分区：
文献类型：
--
作者：
MUNDY, J;HEJGAARD, J;MUNCK, L
为了拓宽大麦种子中基因表达研究的蛋白质选择，进行实验以鉴定其合成在发育和萌发种子组织中受到差异调节的蛋白质。9种不同的大麦蛋白质的体外合成进行了比较，使用mRNA从分离的胚乳和糊粉层组织（发育和成熟的谷物）和培养（发芽）糊粉层脱落酸（阿坝）和GA 3处理。B和C大麦醇溶蛋白多肽和盐溶性蛋白β-淀粉酶、蛋白Z、蛋白C、胰凝乳蛋白酶抑制剂（CI-1和2）、α-淀粉酶抑制剂（CI-1和CI-2）、α-淀粉酶抑制剂（CI-1）、α-淀粉酶抑制剂（CI-2）、α-淀粉酶抑制剂（CI-1）、α-淀粉酶抑制剂（CI-淀粉酶/枯草杆菌蛋白酶抑制剂（ASI）和动物无细胞蛋白质合成系统抑制剂（PSI）是用来自发育中的淀粉胚乳组织的mRNA合成的。在这些蛋白质中，淀粉酶、Z蛋白、CI- 1和CI-2也可由糊粉层发育细胞的mRNA合成，但ASI、PSI和C蛋白则不合成。CI-1和一个可能的淀粉酶/蛋白酶抑制剂（PAPI）的合成在高水平的mRNA从发育后期和成熟糊粉。这些结果表明，编码PAPI和CI-1的mRNA在种子干燥后存活，并且在糊粉层细胞中寿命长。因此，编码ASI、PSI、蛋白C和PAPI的基因的表达在种子发育期间是组织和阶段特异性的。只有ASI，CI-1，和PAPI的合成量显着与mRNA从培养糊粉层。PAPI和CI-1的合成水平与激素处理无关。与此相反，α-β-D-半乳糖苷的合成α-淀粉酶（作为对照包括在内）和ASI的mRNA的积累显示出拮抗性激素控制：而GA促进α-淀粉酶mRNA的积累，阿坝减少α-淀粉酶mRNA的积累。淀粉酶，这些激素对ASI mRNA水平具有相反的作用。
To widen the selection of proteins for gene expression studies in barley seeds, experiments were performed to identify proteins whose synthesis is differentially regulated in developing and germinating seed tissues. The in vitro synthesis of nine distinct barley proteins was compared using mRNAs from isolated endosperm and aleurone tissues (developing and mature grain) and from cultured (germinating) aleurone layers treated with abscisic acid (ABA) and GA3. B and C hordein polypeptides and the salt-soluble proteins .beta.-amylase, protein Z, protein C, the chymotrypsin inhibitors (CI-1 and 2), the .alpha.-amylase/subtilisin inhibitor (ASI) and the inhibitor of animal cell-free protein synthesis systems (PSI) were synthesized with mRNA from developing starchy endosperm tissue. Of these proteins, .beta.-amylase, protein Z, and CI- 1 and 2 were also synthesized with mRNA from developing aleurone cells, but ASI, PSI, and protein C were not. CI-1 and also a probable amylase/protease inhibitor (PAPI) were synthesized at high levels with mRNAs from late developing and mature aleurone. These results show that mRNAs encoding PAPI and CI-1 survive seed dessication and are long-lived in aleurone cells. Thus, expression of genes encoding ASI, PSI, protein C, and PAPI is tissue and stage-specific during seed development. Only ASI, CI-1, and PAPI were synthesized in significant amounts with mRNA from cultured aleurone layers. The levels of synthesis of PAPI and CI-1 were independent of hormone treatment. In contrast, synthesis of .alpha.-amylase (included as control) and of ASI showed antagonistic hormonal control: while GA promotes and ABA reduces accumulation of mRNA for .alpha.-amylase, these hormones have the opposite effect on ASI mRNA levels.