DIFFERENTIAL EFFECT OF SELF-STIMULATION ON DOPAMINE RELEASE AND METABOLISM IN THE RAT MEDIAL FRONTAL-CORTEX, NUCLEUS-ACCUMBENS AND STRIATUM STUDIED BY INVIVO MICRODIALYSIS
DIFFERENTIAL EFFECT OF SELF-STIMULATION ON DOPAMINE RELEASE AND METABOLISM IN THE RAT MEDIAL FRONTAL-CORTEX, NUCLEUS-ACCUMBENS AND STRIATUM STUDIED BY INVIVO MICRODIALYSIS
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DOI：
10.1016/0006-8993(92)90813-o
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发表时间：
1992-03-06
期刊：
影响因子：
2.9
通讯作者：
NAGATSU, T
中科院分区：
文献类型：
--
作者：
NAKAHARA, D;FUCHIKAMI, K;NAGATSU, T
用微透析法测定了多巴胺（DA）摄取抑制剂诺米芬辛（1 mg/kg，ip）预处理大鼠内侧前脑束（MFB）自我刺激过程中，多巴胺能终末区内侧额叶皮质（MFC）、纹状体（STR）、中脑核（NAC）细胞外多巴胺（DA）及其代谢产物水平的变化。在诺米芬辛预处理的大鼠中，MFB的自我刺激引起MFC和NAC中细胞外DA水平的增加，但不引起STR中的增加。自我刺激也使MFC和NAC中主要DA代谢物3，4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）的细胞外浓度增加到相似的程度，并在STR中增加到较小的程度。用MFC或NAC自我刺激后，神经化学变化存在区域差异，显示DA、DOPAC和HVA的细胞外水平高于STR。此外，在刺激的同侧和对侧两个半球上都观察到这些变化。结果表明，MFB的自我刺激优先激活中皮质边缘DA系统，从而双边增加DA的释放及其代谢产生在其终端区域，MFC和NAC。
Changes in the extracellular levels of dopamine (DA) and its metabolites in the dopaminergic terminal regions, the medial frontal cortex (MFC), nucleus accumbens (NAC), and striatum (STR), were measured by microdialysis during self-stimulation of the medial forebrain bundle (MFB) in rats pretreated with the DA uptake inhibitor, nomifensine (1 mg/kg, i.p.). Self-stimulation of the MFB in nomifensine-pretreated rats caused an increase in the extracellular DA level in the MFC and NAC but not in the STR. Self-stimulation also increased the extracellular concentrations of the main DA metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) to a similar extent in the MFC and NAC and to a lesser extent in the STR. Thus, there was a regional difference in the neurochemical changes following self-stimulation with either the MFC or the NAC showing larger extracellular levels of DA, DOPAC, and HVA than the STR. Furthermore, these changes were observed on both hemispheres ipsilateral and contralateral to the stimulation. The results indicate that self-stimulation of the MFB preferentially activates the mesocorticolimbic DA systems, thereby bilateral increases in the release of DA and its metabolism being produced in their terminal regions, the MFC and NAC.