Effect of cadmium, copper and mercury on antioxidant enzyme activities and lipid peroxidation in the gills of the hydrothermal vent mussel Bathymodiolus azoricus
Effect of cadmium, copper and mercury on antioxidant enzyme activities and lipid peroxidation in the gills of the hydrothermal vent mussel Bathymodiolus azoricus
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DOI：
10.1016/j.marenvres.2004.03.083
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发表时间：
2004-08-01
影响因子：
3.3
通讯作者：
Fiala-Médioni, A
中科院分区：
文献类型：
--
作者：
Company, R;Serafim, A;Fiala-Médioni, A
众所周知，金属会影响海洋生物的脂质过氧化和氧化状态。热液喷口贻贝生活在深海环境中，条件异常，包括金属浓度高。固氮芽孢杆菌虽然是好氧生物，但它们的鳃中含有丰富的甲烷和硫自养共生菌。研究了镉(0.9 um)、铜(0.4 um)和汞(0.1 um)不同暴露时间对固氮鱼(Razoricus)鳃组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、总谷胱甘肽过氧化物酶(总GPx)和硒依赖谷胱甘肽过氧化物酶(Se-GPx)的保护作用及脂质过氧化作用的影响。实验在加压容器中进行，温度为9+/-1℃，气压为85巴。结果表明，文华贻贝在鳃中具有抗氧化酶保护作用。镉和铜对酶防御系统有抑制作用，而对汞则相反。在B.azoricus中，这些酶系统并不完全被理解，因为由于存在硫化氢和氧含量，活性氧物种可能通过自然氧化还原循环以外的其他过程产生。此外，共生细菌可能在鳃的抗氧化保护中发挥重要作用。(C)2004爱思唯尔有限公司。保留所有权利。
Metals are known to influence lipid peroxidation and oxidative status of marine organisms. Hydrothermal vent mussels Bathymodiolus azoricus live in deep-sea environments with anomalous conditions, including high metal concentrations. Although B. azoricus are aerobic organisms they possess abundant methano and thioautotrophic symbiotic bacteria in the gills. The enzymatic defences (superoxide dismutase (SOD), catalase (CAT), total glutathione peroxidase (Total GPx) and selenium-dependent glutathione peroxidase (Se-GPx)) and lipid peroxidation were determined in the gills of R azoricus exposed to Cd (0.9 muM), Cu (0.4 muM) and Hg (0.1 muM) with different times of exposure. The experiments were performed in pressurized containers at 9 +/- 1 degreesC and 85 bars.Results show that vent mussels possess antioxidant enzymatic protection in the gills. Cd and Cu had an inhibitory effect in the enzymatic defence system, contrarily to Hg. These enzymatic systems are not completely understood in the B. azoricus, since reactive oxygen species might be produced through other processes than natural redox cycling, due to hydrogen sulphide and oxygen content present. Also the symbiotic bacteria may play an important contribution in the antioxidant protection of the gills. (C) 2004 Elsevier Ltd. All rights reserved.