Amyloid Polypeptide Disaggregation Activity of Passion Fruit Seed-Derived Polyphenol Compounds
Amyloid Polypeptide Disaggregation Activity of Passion Fruit Seed-Derived Polyphenol Compounds
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DOI：
10.1177/1934578x221092710
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发表时间：
2022-09
影响因子：
1.8
通讯作者：
Tatsuya Sampei;Yingxue Wu;H. Shigemori
中科院分区：
文献类型：
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作者：
Tatsuya Sampei;Yingxue Wu;H. Shigemori
在老龄化社会，阿尔茨海默病(AD)和2型糖尿病(T2D)的患病率有所增加。目前认为这些疾病是由淀粉样蛋白(Aβ，Aβ)在脑内聚集和人胰岛淀粉样多肽(HIAPP)在郎格汉斯胰岛聚集引起的。因此，解聚这些现有的淀粉样蛋白聚集体是预防和治疗这两种疾病的一种有前途的方法。在我们以前的研究中，我们发现含儿茶酚基团的多酚类化合物具有显著的Aβ和hIAPP聚集抑制活性。与以往关于它们的聚集抑制活性的报道相比，关于多酚类化合物的解聚活性的报道很少。此外，关于多酚类化合物在hIAPP上的解聚活性的研究很少。在这项研究中，我们研究了西番莲种子中发现的多酚化合物玄参素B及其相关化合物的Aβ和hIAPP解聚活性。用硫代黄素T(Th-T)分析和透射电子显微镜研究了它们的Aβ42和hIAPP解聚活性。结果表明，玄参素B及其相关化合物具有显著的解聚活性。这些化合物的构效关系表明，邻苯二酚基团的存在对该活性很重要。本研究还表明，西番莲种子多酚对淀粉样多肽聚集有显著的解聚活性。
In an aging society, the prevalence of Alzheimer disease (AD) and type 2 diabetes (T2D) has increased. It is currently hypothesized that these diseases are caused by the aggregation of amyloid β (Aβ) in the brain and human islet amyloid polypeptide (hIAPP) in the islets of Langerhans, respectively. Therefore, the disaggregation of these existing amyloid aggregates is a promising approach to the prevention and treatment of both diseases. In our previous studies, we found a remarkable Aβ and hIAPP aggregation inhibitory activity of polyphenolic compounds containing catechol moieties. Compared to previous reports on their aggregation inhibitory activity, there are few on the disaggregation activity of polyphenolic compounds. Additionally, there are few findings on the disaggregation activity of polyphenolic compounds on hIAPP. In this study, we investigated the Aβ and hIAPP disaggregation activity of scirpusin B, a polyphenolic compound found in passion fruit seeds, and related compounds. Thioflavin T (Th-T) assays and transmission electron microscopy (TEM) were performed on these compounds to evaluate their Aβ42 and hIAPP disaggregation activities. The results showed that scirpusin B and its related compounds showed remarkable disaggregation activity. The structure–activity relationship of these compounds revealed that the presence of catechol moieties is important for this activity. This study also showed that polyphenols from passion fruit seeds have significant disaggregation activity against amyloid polypeptide aggregation.