Differential expression of S100A2 and S100A4 during progression of human prostate adenocareinoma
Differential expression of S100A2 and S100A4 during progression of human prostate adenocareinoma
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DOI：
10.1200/jco.2003.03.024
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发表时间：
2003-01-01
影响因子：
45.3
通讯作者：
Mukhtar, H
中科院分区：
文献类型：
--
作者：
Gupta, S;Hussain, T;Mukhtar, H
目的：为了建立钙结合蛋白S10 OA 2和S10 OA 4在人前列腺腺癌进展过程中的临床意义，患者和方法：在正常人前列腺上皮细胞（NHPE）、病毒转化的前列腺上皮细胞（NHPE）和正常人前列腺上皮细胞（NHPE）中测定S10 OA 2和S10 OA 4的表达模式。（PZ-HPV-7）;几种人前列腺癌细胞（22 Rv 1、DU 145、LNCaP和PC 3）;在经尿道前列腺切除术期间从患有良性前列腺增生（BPH）、前列腺炎和腺癌的患者获得的组织样品;结果：S10 OA 2蛋白在NHPE和PZ-HPV-7细胞中高表达，而在22 Rv 1、DU 145、LNCaP和PC 3细胞中完全缺失。BPH和前列腺炎的组织样本显示出比低级别癌症（Gleason评分小于或等于6）更高的S100 A2 mRNA和蛋白水平，而在高级别癌症样本中观察到完全丧失（Gleason评分> 6）。免疫组化分析进一步证实了S10 OA 2在良性组织中的高水平，并且随着肿瘤分级的增加而逐渐丢失。S100 A4蛋白在所有癌细胞中的表达水平均显著高于NHPE和PZ-HPV-7细胞。S100 A4的mRNA和蛋白水平在高级别癌症标本中显著高于BPH、前列腺炎和低级别癌症。结论：S100 A2的缺失和S100 A4的表达增加可能是前列腺癌进展过程中的重要事件。(C)2003年，美国临床肿瘤学会。
Purpose : To establish the clinical significance of calcium binding proteins S10OA2 and S10OA4 during progression of human prostate adenocarcinoma.Patients and Methods: Expression pattern of S10OA2 and S10OA4 was determined in normal human prostate epithelial cells (NHPE); virally transformed prostate epithelial cells (PZ-HPV-7); several human prostate carcinoma cells (22Rv1, DU145, LNCaP, and PC3); tissue samples obtained during transuretheral prostatic resection from patients with benign prostate hyperplasia (BPH), prostatitis, and adenocarcinorna; and paraffin-embedded sections from pair-matched benign and cancer specimens of different tumor grade.Results : High constitutive protein expression of S10OA2 was observed in NHPE and PZ-HPV-7 cells, whereas its complete absence was observed in 22Rv1, DU145, LNCaP, and PC3 cells. Tissue samples of BPH and prostatitis exhibited higher mRNA and protein levels of S10OA2 than lowgrade cancer (Gleason score less than or equal to 6), whereas a complete loss was observed in high-grade cancer specimens (Gleason score > 6). Immunohistochernical analysis further confirmed high levels of S10OA2 in benign tissues and a progressive loss with increasing tumor grade. The protein level of S100A4 was significantly higher in all carcinoma cells compared with NHPE and PZ-HPV-7 cells. The mRNA and protein level of S100A4 was significantly higher in high-grade cancer specimens compared with BPH, prostatitis, and lowgrade cancer. The high levels of S100A4 observed in cancer tissue correlated with increasing tumor grade.Conclusion: Loss of S10OA2 and increased expression of S100A4 may be an important event during progression of prostate cancer in humans. (C) 2003 by American Society of Clinical Oncology.