Raman spectroscopic detection of changes in molecular composition of bladder muscle tissue caused by outlet obstruction
Raman spectroscopic detection of changes in molecular composition of bladder muscle tissue caused by outlet obstruction
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DOI：
10.1016/s0924-2031(03)00047-x
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发表时间：
2003-08-05
影响因子：
2.5
通讯作者：
Puppels, GJ
中科院分区：
文献类型：
--
作者：
de Jong, BWD;Schut, TCB;Puppels, GJ
膀胱出口梗阻会因结构损伤而导致膀胱功能丧失。梗阻膀胱的功能参数无法预测梗阻清除后的临床结果。因此，需要其他诊断方法。这项研究提出了基于拉曼光谱的方法的初步结果，该方法旨在检测可能具有诊断价值的膀胱壁分子组成的变化。使用膀胱梗阻豚鼠模型中受损和未受损膀胱壁的未固定部分的拉曼光谱图来检测膀胱肌肉组织成分的变化。肌肉纤维中的胶原蛋白浸润清晰可见。所揭示的其他成分变化包括梗阻膀胱壁中糖原的积累以及蛋白质成分的明显但迄今未知的变化。体内拉曼光谱应用可以无需活检即可确定膀胱结构。这些初步发现表明拉曼光谱可以成为评估膀胱结构损失程度的有价值的诊断工具。 (C) 2003 Elsevier Science B.V. 保留所有权利。
Bladder outlet obstruction leads to loss of bladder function as a result of structural damage. Functional parameters of an obstructed bladder do not enable a prediction of the clinical outcome of removal of the obstruction. Therefore, other diagnostic methods are needed. This study presents first results of an approach based on Raman spectroscopy, which aims to detect changes in molecular composition of the bladder wall that may have diagnostic value. Raman spectroscopic mapping of unfixed sections of damaged and undamaged bladder wall from a guinea pig model of bladder obstruction was used to detect changes in composition of bladder muscle tissue. Collagen infiltration in muscle fibers was clearly visualized. Other compositional changes that are revealed include the accumulation of glycogen in obstructed bladder wall as well as an apparent but as yet unknown change in protein composition. In vivo Raman spectroscopic application may enable determination of bladder structure without the need for biopsies. These initial findings show that Raman spectroscopy can be a valuable diagnostic tool for evaluation of the extent of bladder structure loss. (C) 2003 Elsevier Science B.V. All rights reserved.