Effects of interferon-alpha (IFN-alpha) administration on leucocytes in healthy humans
Effects of interferon-alpha (IFN-alpha) administration on leucocytes in healthy humans
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DOI：
10.1111/j.1365-2249.1997.269-ce1161.x
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发表时间：
1997-02-01
影响因子：
4.6
通讯作者：
Romijn, JA
中科院分区：
文献类型：
--
作者：
Corssmit, EPM;Heijligenberg, R;Romijn, JA
IFN-α的血浆浓度在几种炎性疾病中增加。一些证据表明IFN-α具有抗炎特性。为了研究IFN-α对白细胞亚群和活化以及细胞因子的影响，我们在一项随机对照交叉研究中向8名健康受试者给予IFN-α（rhIFN-α 2b; 5 × 10（6）U/m（2）），并分析了循环白细胞以及中性粒细胞和单核细胞活化参数的变化。IFN-α给药后，中性粒细胞计数升高，单核细胞计数一过性降低，而淋巴细胞、嗜碱性粒细胞和嗜酸性粒细胞的数量显示持续降低。IFN-α给药也与中性粒细胞活化相关，反映在弹性蛋白酶-α（1）-抗胰蛋白酶复合物和乳铁蛋白的血浆浓度增加。血清新蝶呤，单核细胞活化的标志物，显着增加10小时后，管理的IFN-α。IFN-α显著增加IL-6、IL-8和IL-10的血浆浓度。虽然IL-1和肿瘤坏死因子（TNF）仍然检测不到，可溶性TNF受体p55和p75的血浆浓度增加后，IFN-α管理。我们得出结论，IFN-α诱导白细胞的分布和功能特性的多种改变。IFN-α在细胞因子网络中发挥促炎和抗炎作用。
Plasma concentrations of IFN-alpha are increased in several inflammatory conditions. Several lines of evidence indicate that IFN-alpha has anti-inflammatory properties. To study the effects of IFN-alpha on leucocyte subsets and activation and on cytokines, we administered IFN-alpha (rhIFN-alpha 2b; 5 x 10(6) U/m(2)) to eight healthy human subjects in a randomized controlled cross-over study and analysed changes in circulating leucocytes and parameters for neutrophil and monocyte activation. After administration of IFN-alpha, neutrophil counts increased, monocyte counts decreased transiently, whereas the number of lymphocytes, basophils and eosinophils showed a sustained decrease. IFN-alpha administration was also associated with neutrophil activation, reflected in an increase in the plasma concentrations of elastase-alpha(1)-antitrypsin complexes and lactoferrin. Serum neopterin, a marker for monocyte activation, was significantly increased 10 h after administration of IFN-alpha. IFN-alpha significantly increased plasma concentrations of IL-6, IL-8 and IL-10. Although IL-1 and tumour necrosis factor (TNF) remained undetectable, plasma concentrations of soluble TNF receptors p55 and p75 increased after IFN-alpha administration. We conclude that IFN-alpha induces multiple alterations in the distribution and functional properties of leucocytes. IFN-alpha exerts pro- as well as anti-inflammatory effects within the cytokine network.