ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1-and SRE-mediated transcriptional activity
ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1-and SRE-mediated transcriptional activity
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ZNF552 是一种新型人 KRAB/C2H2 锌指蛋白，可抑制 AP-1 和 SRE 介导的转录活性
DOI：
10.5483/bmbrep.2010.43.3.193
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发表时间：
2010-03-31
期刊：
影响因子：
3.8
通讯作者：
Yuan, Wuzhou
中科院分区：
文献类型：
--
作者：
Deng, Yun;Liu, Bisheng;Yuan, Wuzhou
在这项研究中，我们报告了一个新的C2 H2锌指蛋白，ZNF 552，从人胚胎心脏cDNA文库的鉴定和表征。ZNF 552由3个外显子和2个内含子组成，定位于染色体19q13.43。ZNF 552的cDNA全长2.3 kb，编码407个氨基酸，在细胞核和细胞质中含有一个氨基端KRAB结构域和7个羧基端C2 H2锌指结构域。北方印迹分析表明，ZNF 552在肝、肺、脾、睾丸和肾组织中均有表达，其中在成年人的肺和睾丸组织中表达量较高。报告基因分析表明，ZNF 552是一个转录抑制因子，ZNF 552在COS-7细胞中的过表达抑制了AP-1和SRE的转录活性，RNAi分析表明ZNF 552的过表达抑制了AP-1和SRE的转录活性。缺失研究表明，ZNF 552的KRAB结构域可能参与这种抑制作用。[BMB报告2010; 43（3）：193-198]
In this study, we report the identification and characterization of a novel C2H2 zinc finger protein, ZNF552, from a human embryonic heart cDNA library. ZNF552 is composed of three exons and two introns and maps to chromosome 19q13.43. The cDNA of ZNF552 is 2.3 kb, encoding 407 amino acids with an amino-terminal KRAB domain and seven carboxyl-terminal C2H2 zinc finger motifs in the nucleus and cytoplasm. Northern blotting analysis indicated that a 2.3 kb transcript specific for ZNF552 was expressed in liver, lung, spleen, testis and kidney, especially with a higher level in the lung and testis in human adult tissues. Reporter gene assays showed that ZNF552 was a transcriptional repressor, and overexpression of ZNF552 in the COS-7 cells inhibited the transcriptional activities of AP-1 and SRE, which could be relieved through RNAi analysis. Deletion studies showed that the KRAB domain of ZNF552 may be involved in this inhibition. [BMB reports 2010; 43(3): 193-198]