A comparative analysis of three classes of bacterial non-specific Acid phosphatases and archaeal phosphoesterases: evolutionary perspective.
A comparative analysis of three classes of bacterial non-specific Acid phosphatases and archaeal phosphoesterases: evolutionary perspective.
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DOI：
10.5455/aim.2012.20.167-173
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发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
B Chandra S
中科院分区：
文献类型：
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作者：
U Gandhi N;B Chandra S
细菌非特异性酸性磷酸水解酶（NSAPs）或磷酸酶是一组作为可溶性周质蛋白分泌或作为膜结合脂蛋白保留的酶，其通常能够使广泛的结构上不相关的有机磷酸酯（核苷酸、糖磷酸盐、植酸等）去磷酸化。以获得无机磷酸盐（Pi）和有机副产物。它们在酸性至中性pH值下表现出最佳的催化活性。根据氨基酸序列的相关性，将磷酸酶分为不同的分子家族，即A类、B类和C类酸性磷酸酶。在这篇文章中的33个序列，26属于每一类的细菌酸性磷酸酶和7属于古细菌的磷酸酯酶的生物信息学的各种工具进行了分析。系统发育分析，点图比较和模体分析，以确定一些相似性和差异的3类细菌的酸性磷酸酶和古细菌的磷酸酯酶。本研究利用生物信息学的方法对三类细菌酸性磷酸酶和古细菌磷酸酯酶的进化关系进行了研究。
Bacterial nonspecific acid phosphohydrolases (NSAPs) or phosphatases are group of enzymes secreted as soluble periplasmic proteins or retained as membrane bound lipoproteins that are usually able to dephosphorylate a broad array of structurally unrelated organic phosphoesters (nucleotides, sugar phosphates, phytic acid etc.) to acquire inorganic phosphate (Pi) and organic byproducts. They exhibit optimal catalytic activity at acidic to neutral pH values. On the basis of amino acid sequence relatedness, phosphatase are grouped into different molecular families namely Class A, Class B and Class C acid phosphatase respectively. In this article out of thirty three sequences, twenty six belonging to each of the three classes of bacterial acid phosphatase and seven belonging to archaeal phosphoesterases were analyzed using various tools of bioinformatics. Phylogenetic analysis, dot plot comparisons and motif analysis were done to identify a number of similarities and differences between three classes of bacterial acid phosphatases and archaeal phosphoesterases. In this research we have attempted to decipher evolutionary relationship between three classes of bacterial acid phosphatase and archaeal phosphoesterases using bioinformatics approach.