Estrogen Formation by the Isolated Perfused Rhesus Monkey Brain
Estrogen Formation by the Isolated Perfused Rhesus Monkey Brain
复制标题
离体灌注恒河猴脑形成雌激素
作者：
F. Flores;F. Naftolin;K. Ryan;R. White
用[3H]雄烯二酮灌注未成熟雄性恒河猴的两个离体脑，从灌注液中鉴定出游离和结合的[3H]雌酮和游离[3H]雌二醇。在解剖的脑组织中，雌激素仅从下丘脑和边缘系统中回收。在这两个实验中，在40分钟的灌注过程中，雄烯二酮产生的雌激素总计为1.58和2.83纳克。
Perfusion of two isolated brains from immature male rhesus monkeys with [3H]androstenedione resulted in the identification of free and conjugated [3H]estrone and free [3H]estradiol from the perfusates. In the dissected cerebral tissues, estrogens were recovered only from the hypothalamus and limbic system. The production of estrogens from androstenedione during the 40-minute perfusions in these two experiments totaled 1.58 and 2.83 nanograms.