The potential efficacy of 3,3′-diindolylmethane in prevention of prostate cancer development
The potential efficacy of 3,3′-diindolylmethane in prevention of prostate cancer development
复制标题
DOI：
10.1097/cej.0b013e328333fbce
复制
发表时间：
2010-05-01
影响因子：
2.4
通讯作者：
Stein, Avi
中科院分区：
文献类型：
--
作者：
Fares, Fuad;Azzam, Naiel;Stein, Avi
本研究的目的是在动物模型中检查3，3'-二吲哚基甲烷（DIM）在预防前列腺癌肿瘤发展中的功效。将小鼠前列腺癌细胞（TRAMP-C2，2 × 10（-6））皮下注射到三组C57 BL/6小鼠中（每组10只小鼠）。两组在早期用DIM处理;各2或10 mg/kg，另外一个对照组注射培养基。动物再处理5周直至处死。每两周测量一次肿瘤大小。在实验结束时，处死小鼠，切除肿瘤，称重，测量并使用免疫组织化学研究进行测试。此外，采集血样进行生化分析。结果表明，与对照组相比，DIM显著降低了治疗动物的肿瘤发展。80%的对照组动物以及40%和60%的10或2 mg/kg DIM给药组动物分别发生肿瘤。此外，在治疗的动物中发展的肿瘤显著（P
The objective of this study was to examine the efficacy of 3,3'-diindolylmethane (DIM) in prevention of prostate cancer tumor development in an animal model. Mouse prostate cancer cells (TRAMP-C2, 2 x 10(-6)) were injected subcutaneously into three groups of C57BL/6 mice (10 mice in each group). Two groups were treated earlier with DIM; 2 or 10 mg/kg each, and an additional control group was injected with medium. Animals were treated for five more weeks until sacrificed. Tumor sizes were measured biweekly. At the end of the experiment, mice were sacrificed, and tumors were excised, weighed, measured and tested using immunohistochemical studies. In addition blood samples were collected for biochemical analysis. The results indicated that DIM significantly reduced tumor development in treated animals when compared with controls. Tumors developed in 80% of controls and 40% and 60% of animals treated with 10 or 2 mg/kg of DIM, respectively. Moreover, tumors that developed in treated animals were significantly (P