Social cues from conspecifics alter electrical activity of gonadotropin-releasing hormone neurons in the terminal nerve via visual signals
Social cues from conspecifics alter electrical activity of gonadotropin-releasing hormone neurons in the terminal nerve via visual signals
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DOI：
10.1152/ajpregu.00143.2009
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发表时间：
2009-07-01
影响因子：
2.8
通讯作者：
Wayne, Nancy L.
中科院分区：
文献类型：
--
作者：
Ramakrishnan, Siddharth;Wayne, Nancy L.
罗摩克里希南 S，韦恩 NL。来自同种动物的社交线索通过视觉信号改变终末神经中促性腺激素释放激素神经元的电活动。 Am J Physiol Regul Integr Comp Physiol 297：R135-R141，2009。首次发表于 2009 年 5 月 6 日； doi:10.1152/ajpregu.00143.2009.-脊椎动物大脑中存在多种促性腺激素释放激素 (GnRH) 神经元。位于下丘脑/视前区的群体是研究最充分的，并且已知最终控制繁殖。硬骨鱼类在与嗅球相关的终末神经 (TN) 中具有额外的 GnRH 神经元群，其生理功能仍不清楚。解剖学和生理学研究提供的证据表明 TN-GnRH 神经元在大脑中具有广泛的投射并调节神经元活动。尽管有解剖学证据表明 TN 接收嗅觉和视觉感觉输入，但尚不清楚感觉信息是否传输到 TN-GnRH 神经元以调节其活动。在本研究中，我们测试了以下假设：来自同种动物的社交线索调节雌性青鳉鱼（Oryzias latipes）完整大脑中 TN-GnRH 神经元的电活动。我们进一步研究了化学感应和视觉信号在调节社交线索反应中的潜在作用。我们使用了带有绿色荧光蛋白基因标记的 TN-GnRH 神经元的转基因青鳉品系，从而可以在全细胞电流钳电生理学中对特定神经元进行可视化。我们证明，与暴露于其他雌性相比，24 小时暴露于雄性视觉和化学感觉信号会抑制雌性 TN-GnRH 神经元的电活动。仅化学感应线索不足以诱导这种社交线索反应。然而，仅视觉线索就复制了“组合的”社交线索反应。这些发现支持我们的假设，即感觉信号，特别是视觉社交线索调节 TN-GnRH 神经元的电活动。
Ramakrishnan S, Wayne NL. Social cues from conspecifics alter electrical activity of gonadotropin-releasing hormone neurons in the terminal nerve via visual signals. Am J Physiol Regul Integr Comp Physiol 297: R135-R141, 2009. First published May 6, 2009; doi:10.1152/ajpregu.00143.2009.-There are multiple populations of gonadotropin-releasing hormone (GnRH) neurons in the brains of vertebrates. The population located in the hypothalamus/preoptic area is the best studied and is known to ultimately control reproduction. Teleost fish have an additional population of GnRH neurons in the terminal nerve (TN) associated with the olfactory bulbs, the physiological function of which is still unclear. Anatomical and physiological studies provide evidence that TN-GnRH neurons have extensive projections in the brain and modulate neuronal activity. Although there is anatomical evidence that the TN receives olfactory and optic sensory inputs, it is not known if sensory information is transmitted to TN-GnRH neurons to modulate their activity. In the present study, we tested the hypothesis that social cues from conspecifics modulate electrical activity of TN-GnRH neurons from the intact brain of female medaka fish (Oryzias latipes). We further investigated the potential roles of chemosensory and visual signals in mediating the social cue response. We used a transgenic line of medaka with TN-GnRH neurons genetically tagged with green fluorescent protein, allowing visualization of specific neurons for whole-cell current clamp electrophysiology. We demonstrated that 24-h exposure to male visual and chemosensory cues suppressed the electrical activity of female TN-GnRH neurons compared with exposure to other females. Chemosensory cues alone were insufficient to induce this social cue response. However, visual cues alone replicated the "combined" social cue response. These findings support our hypothesis that sensory signals-and specifically, visual social cues modulate electrical activity of TN-GnRH neurons.