CELL-SPECIFIC IMMUNOPROBES FOR THE BRAIN OF NORMAL AND MUTANT DROSOPHILA-MELANOGASTER .1. WILDTYPE VISUAL-SYSTEM
CELL-SPECIFIC IMMUNOPROBES FOR THE BRAIN OF NORMAL AND MUTANT DROSOPHILA-MELANOGASTER .1. WILDTYPE VISUAL-SYSTEM
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DOI：
10.1007/bf00222292
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发表时间：
1988-01-01
影响因子：
3.6
通讯作者：
OERTEL, WH
中科院分区：
文献类型：
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作者：
BUCHNER, E;BADER, R;OERTEL, WH
我们在果蝇大脑视叶中筛选了用于免疫细胞化学染色的抗体。描述了七种多克隆抗血清和五种单克隆抗体，它们选择性地和可重复地对单个细胞进行染色和/或在神经堆中产生特征性染色模式。这种抗血清可用于视觉突变体中分子和结构脑缺陷的细胞表征。在野生型视觉系统中，我们目前可以单独染色以下内容：柱状“T 1”神经元的整个补充；一小组推定的血清素能神经元；约 3000 个细胞含有并合成 γ-氨基丁酸 (GABA)；以及三组将抗体与 Ca2+ 结合蛋白结合的细胞。此外，通过使用黑腹果蝇大脑匀浆免疫小鼠后产生的单克隆抗体，已经鉴定出迄今为止未知的小群切向细胞，这些细胞将精细的树枝状结构发送到髓质的特定层中，以及视觉神经丛中的两种特征层模式。
We have screened antibodies for immunocytochemical staining in the optic lobes of the brain ofDrosophila melanogaster. Seven polyclonal antisera and five monoclonal antibodies are described that selectively and reproducibly stain individual cells and/or produce characteristic staining patterns in the neuropile. Such antisera are useful for the cellular characterization of molecular and structural brain defects in visual mutants. In the wildtype visual system we can at present separately stain the following: the entire complement of columnar “ T 1” neurons; a small set of presumptive serotonergic neurons; some 3000 cells that contain and synthesizeγ-amino butyric acid (GABA); and three groups of cells that bind antibodies to Ca2+-binding proteins. In addition, small groups of hitherto unknown tangential cells that send fine arborizations into specific strata of the medulla, and two patterns of characteristic layers in the visual neuropile have been identified by use of monoclonal antibodies generated following immunization of mice with homogenates of the brain ofDrosophila melanogaster.