A monoclonal antibody to P-selectin ameliorates injury associated with hemorrhagic shock in rabbits.
A monoclonal antibody to P-selectin ameliorates injury associated with hemorrhagic shock in rabbits.
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P-选择素单克隆抗体可改善兔子失血性休克相关损伤。
DOI：
10.1152/ajpheart.1994.267.6.h2391
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Harlan,JM
中科院分区：
文献类型：
--
作者：
Winn,RK;Paulson,JC;Harlan,JM
P-选择素（CD 62 P）表达于内皮细胞表面，参与白细胞滚动。我们研究了CD 62 P在失血性休克和复苏中的作用。用生理盐水、抗CD 62 P（α-CD 62 P）单克隆抗体（MAb; PB1.3）、同种型匹配MAb（PNB 1.6）或α-CD 18 MAb（60.3）处理家兔。将导管置于股动脉和静脉中进行监测，然后将心输出量降至基线的33%，持续1.5 h。回输失血，并继续复苏，以将心输出量维持在基线的90%以内。生理盐水和MAb PNB 1.6给药组之间无统计学差异;因此将其合并为对照组。MAb PB1.3和MAb 60.3处理组需要的液体复苏显著少于对照组（分别为12、17和56 ml/kg）。我们的结论是，针对CD 62 P或CD 18功能表位的单克隆抗体对失血性休克后的血管损伤提供了显着的保护。
P-selectin (CD62P) can participate in leukocyte rolling when it is expressed on the surface of endothelial cells. We examined the role of CD62P in hemorrhagic shock and resuscitation. Rabbits were treated with saline, an anti-CD62P (alpha-CD62P) monoclonal antibody (MAb; PB1.3), an isotype-matched MAb (PNB1.6), or an alpha-CD18 MAb (60.3). Catheters were placed in the femoral artery and vein for monitoring, and cardiac output was then reduced to 33% of baseline for 1.5 h. Shed blood was returned, and resuscitation was continued to maintain the cardiac output to within 90% of baseline. There were no statistical differences between the saline- and MAb PNB1.6-treated groups; they were therefore combined into a control group. The MAb PB1.3- and MAb 60.3-treated groups required significantly less fluid resuscitation than the controls (12, 17, and 56 ml/kg, respectively). We conclude that MAbs directed to functional epitopes of either CD62P or CD18 provide significant protection from vascular injury after hemorrhagic shock.