Phenotypic instability of Saos-2 cells in long-term culture
Phenotypic instability of Saos-2 cells in long-term culture
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DOI：
10.1016/j.bbrc.2005.05.097
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发表时间：
2005-07-22
影响因子：
3.1
通讯作者：
Brenner, RE
中科院分区：
文献类型：
--
作者：
Hausser, HJ;Brenner, RE
人类成骨肉瘤细胞系Saos-2被广泛用作人类成骨细胞的模型系统，尽管其表型稳定性尚未确定。因此，我们将这些细胞繁殖超过100代，并比较了相关的表型特性。一般来说，高传代细胞具有较高的增殖率和较低的特异性碱性磷酸酶活性，尽管矿化在培养的晚传代细胞中更为明显。虽然大多数基因的表达变化不大，但有些基因表现出显著差异。例如，已被讨论为增殖和矿化调节剂的Decorin仅在早期传代细胞中强烈表达，而多营养因子和midkine的两种受体分别在早期和晚期传代细胞中表现出几乎互斥的表达模式。我们的观察表明，在比较不同培养历史的Saos-2细胞获得的结果时需要特别小心。(c) 2005爱思唯尔公司版权所有。
The human osteosarcoma cell line Saos-2 is widely used as a model system for human osteoblastic cells, though its phenotypic stability has not been ascertained. We therefore propagated these cells over 100 passages and compared relevant phenotypic properties. In general, higher passage cells exhibited higher proliferation rates and lower specific alkaline phosphatase activities, though mineralization was significantly more pronounced in cultures of late passage cells. Whereas expression of most genes investigated did not vary profoundly, some genes exhibited remarkable differences. Decorin, for instance, that has been discussed as a regulator of proliferation and mineralization, is strongly expressed only in early passage cells, and two receptors for pleiotrophin and midkine exhibited an almost mutually exclusive expression pattern in early and late passage cells, respectively. Our observations indicate that special care is required when results obtained with Saos-2 cells with different culture history are to be compared. (c) 2005 Elsevier Inc. All rights reserved.