Molecular Methods To Improve Diagnosis and Identification of Mucormycosis
Molecular Methods To Improve Diagnosis and Identification of Mucormycosis
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DOI：
10.1128/jcm.00256-11
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发表时间：
2011-06-01
影响因子：
9.4
通讯作者：
Marty, Francisco M.
中科院分区：
文献类型：
--
作者：
Hammond, Sarah P.;Bialek, Ralf;Marty, Francisco M.
毛霉菌病很难诊断。来自疑似病例的样本通常不能在微生物培养中生长毛霉目。我们确定了2001年至2009年在布里格姆妇女医院/丹娜-法伯癌症研究所诊断为毛霉菌病的所有血液恶性肿瘤和干细胞移植患者。对福尔马林固定石蜡包埋的组织样品进行靶向毛霉目18 S核糖体DNA的半巢式PCR和测序。在29例毛霉菌病中，27例有组织样本可用于PCR和测序。毛霉PCR阳性22例。在12例培养阳性的病例中，10例PCR阳性，9例测序结果与培养结果一致。在15例培养阴性的病例中，12例PCR阳性，测序允许属鉴定。毛霉目PCR可用于毛霉菌病的诊断确认，并在培养阴性的情况下进一步表征感染。
Mucormycosis is difficult to diagnose. Samples from suspected cases often fail to grow Mucorales in microbiologic cultures. We identified all hematologic malignancy and stem cell transplant patients diagnosed with proven mucormycosis between 2001 and 2009 at Brigham and Women's Hospital/Dana-Farber Cancer Institute. Seminested PCR targeting Mucorales 18S ribosomal DNA and sequencing were performed on formalin-fixed paraffin-embedded tissue samples. Of 29 cases of mucormycosis, 27 had tissue samples available for PCR and sequencing. Mucorales PCR was positive in 22. Among 12 culture-positive cases, 10 were PCR positive and sequencing was concordant with culture results to the genus level in 9. Among 15 culture-negative cases, PCR was positive and sequencing allowed genus identification in 12. Mucorales PCR is useful for confirmation of the diagnosis of mucormycosis and for further characterization of the infection in cases where cultures are negative.