Expression of adropin in rat brain, cerebellum, kidneys, heart, liver, and pancreas in streptozotocin-induced diabetes
Expression of adropin in rat brain, cerebellum, kidneys, heart, liver, and pancreas in streptozotocin-induced diabetes
复制标题
DOI：
10.1007/s11010-013-1660-4
复制
发表时间：
2013-08-01
影响因子：
4.3
通讯作者：
Kendir, Yalcin
中科院分区：
文献类型：
--
作者：
Aydin, Suleyman;Kuloglu, Tuncay;Kendir, Yalcin
我们研究了糖尿病如何影响大鼠脑、小脑、肾脏、心脏、肝脏和胰腺组织中Adropin（ADR）的表达。糖尿病组大鼠腹腔注射（i. p.）注射溶于0.1 M磷酸盐-柠檬酸盐缓冲液（pH 4.5）中的单剂量60 mg/kg链脲霉素（STZ）。将大鼠保持在标准实验室条件下，温度为21至23 A摄氏度，相对湿度为70%，12小时光照/黑暗循环。用标准市售颗粒饲料喂养动物。10周后，将动物处死。ELISA法检测血清和组织上清液中ADR浓度，免疫组化法检测脑、小脑、肾、心、肝、胰腺组织中ADR的表达。然后对数量进行了比较。与对照组相比，在糖尿病诱导大鼠的脑、小脑、肾脏、心脏、肝脏和胰腺中观察到ADR免疫反应增加。在脑（血管区、软脑膜、神经胶质细胞和神经元）、小脑（神经胶质细胞、浦肯野细胞、血管区和颗粒层）、肾脏（肾小球、肾小管周间质细胞和肾小管周毛细血管内皮细胞）、心脏（内膜、心肌和心外膜）、肝脏（窦细胞）和胰腺（浆液性腺泡）中检测到ADR。用ELISA法测定了这些组织中ADR的浓度（以mg/湿重组织计），结果表明，与对照组大鼠相比，糖尿病大鼠中ADR的水平较高。基于mg/湿重组织的组织ADR水平如下：胰腺>肝脏>肾脏>心脏>脑>小脑组织。有证据表明ADR在大鼠的各种组织中表达，并且其水平在STZ诱导的糖尿病中增加;然而，这种对疾病的病理生理学的影响仍有待了解。
We have investigated how diabetes affects the expression of adropin (ADR) in rat brain, cerebellum, kidneys, heart, liver, and pancreas tissues. The rats in the diabetic group were administered an intraperitoneal (i.p.) injection of a single dose of 60 mg/kg streptozotocin (STZ) dissolved in a 0.1 M phosphate-citrate buffer (pH 4.5). The rats were maintained in standard laboratory conditions in a temperature between 21 and 23 A degrees C and a relative humidity of 70 %, under a 12-h light/dark cycle. The animals were fed a standard commercial pellet diet. After 10 weeks, the animals were sacrified. ADR concentrations in the serum and tissue supernatants were measured by ELISA, and immunohistochemical staining was used to follow the expression of the hormones in the brain, cerebellum, kidneys, heart, liver, and pancreas tissues. The quantities were then compared. Increased ADR immunoreaction was seen in the brain, cerebellum, kidneys, heart, liver, and pancreas in the diabetes-induced rats compared to control subjects. ADR was detected in the brain (vascular area, pia mater, neuroglial cell, and neurons), cerebellum (neuroglial cells, Purkinje cells, vascular areas, and granular layer), kidneys (glomerulus, peritubular interstitial cells, and peritubular capillary endothelial cells), heart (endocardium, myocardium, and epicardium), liver (sinusoidal cells), and pancreas (serous acini). Its concentrations (based on mg/wet weight tissues) in these tissues were measured by using ELISA showed that the levels of ADR were higher in the diabetic rats compared to the control rats. Tissue ADR levels based on mg/wet weight tissues were as follows: Pancreas > liver > kidney > heart > brain > cerebellar tissues. Evidence is presented that shows ADR is expressed in various tissues in the rats and its levels increased in STZ-induced diabetes; however, this effect on the pathophysiology of the disorder remains to be understood.