Known structure, unknown function: An inquiry-based undergraduate biochemistry laboratory course.
Known structure, unknown function: An inquiry-based undergraduate biochemistry laboratory course.
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DOI：
10.1002/bmb.20873
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发表时间：
2015-07
期刊：
影响因子：
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通讯作者：
Mura C
中科院分区：
文献类型：
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作者：
Gray C;Price CW;Lee CT;Dewald AH;Cline MA;McAnany CE;Columbus L;Mura C
本科生物化学实验室课程通常不会为学生提供真实的研究经验，特别是当实验室的明确目的纯粹是教学时。然而，一门以探究和研究为基础的教学实验室课程可以同时传授科学知识，培养学生的研究专长和信心。我们开发了一门为期一年的本科生物化学实验课程，学生通过实验和计算来确定已知三维结构的蛋白质的功能。课程的前半部分是探究式和模块化的设计；学生学习一般的生物化学技术，同时为下学期的研究性实验做准备。学生在第一学期学习了标准的生化方法后，在第二学期独立进行自己的(原创)研究项目。根据不同的衡量标准，这一新课程在学生的表现和自信心方面都有了改善。为了向学生和教师传播教学资源，可在http://biochemlab.org.上免费获取生物化学实验室教育资源©2015作者生物化学和分子生物学教育由Wiley期刊公司代表国际生物化学和分子生物学联盟出版，43(4)：245-262,2015。
Undergraduate biochemistry laboratory courses often do not provide students with an authentic research experience, particularly when the express purpose of the laboratory is purely instructional. However, an instructional laboratory course that is inquiry‐ and research‐based could simultaneously impart scientific knowledge and foster a student's research expertise and confidence. We have developed a year‐long undergraduate biochemistry laboratory curriculum wherein students determine, via experiment and computation, the function of a protein of known three‐dimensional structure. The first half of the course is inquiry‐based and modular in design; students learn general biochemical techniques while gaining preparation for research experiments in the second semester. Having learned standard biochemical methods in the first semester, students independently pursue their own (original) research projects in the second semester. This new curriculum has yielded an improvement in student performance and confidence as assessed by various metrics. To disseminate teaching resources to students and instructors alike, a freely accessible Biochemistry Laboratory Education resource is available at http://biochemlab.org. © 2015 The Authors Biochemistry and Molecular Biology Education published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology, 43(4):245–262, 2015.