Insights into the role of iron in immature rat model of hypoxic-ischemic brain injury.
Insights into the role of iron in immature rat model of hypoxic-ischemic brain injury.
复制标题
铁在未成熟大鼠缺氧缺血性脑损伤模型中作用的见解
DOI：
10.3892/etm.2016.3550
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发表时间：
2016-09
影响因子：
2.7
通讯作者：
Cui H
中科院分区：
文献类型：
--
作者：
Wang ZW;Yang LJ;Ding YX;Chang YZ;Cui H
本研究旨在利用 3 日龄 Sprague Dawley 大鼠，在未成熟大鼠模型中探讨铁在缺氧缺血性脑损伤 (HIBI) 发生和发展中的作用。设立正常对照（NC）组、缺氧缺血（HI）组、贫血组、HI+缺血组、早期铁剂治疗组和晚期铁剂治疗组。大鼠脑组织切片用苏木精和伊红染色并进行病理学评估。测量脑组织中铁含量以及铁调节蛋白2（IRP2）和转铁蛋白受体的mRNA表达水平。通过电子显微镜检查少突胶质细胞和轴突的肌动蛋白、微管、髓磷脂和线粒体的超微结构变化。还观察到脑室周围区域存活的髓鞘和少突胶质细胞的数量。 HI组脑组织病理损伤较NC组明显加重。此外，与 NC 组相比，HI 组脑室周围区域的铁含量较高，存活的少突胶质细胞数量减少。 HI+贫血组和NC组之间铁含量没有观察到显着差异。 HI+贫血组存活少突胶质细胞数量较HI组增加，后期铁剂治疗的HI+贫血组较NC组减少，较HI+贫血组增加。电镜显示 HI + 贫血组的髓鞘数量显着高于 HI 组。与HI组相比,HI+贫血组脑组织中IRP2 mRNA表达量显着降低。结果表明，贫血可能会降低 HI 后大脑铁含量的增加速度。然而，早期发生贫血可能可以预防 HIBI。
This study aimed to investigate the role of iron in the occurrence and development of hypoxic-ischemic brain injury (HIBI) in immature rat models using 3-day-old Sprague Dawley rats. Normal control (NC), hypoxic-ischemic (HI), anemia, HI + ischemia, early iron treatment and late iron treatment groups were established. Rat brain tissue sections were stained with hematoxylin and eosin and pathologically evaluated. Iron content and mRNA expression levels of iron regulatory protein 2 (IRP2) and transferrin receptor in the brain tissues were measured. Ultrastructural changes in the actin, microtubules, myelin and mitochondria of oligodendrocytes and axons were examined by electron microscopy. Numbers of viable myelin sheaths and oligodendrocytes in the periventricular area were also observed. Pathological damage of brain tissue in the HI group was markedly increased compared with that in the NC group. Furthermore, there was a higher iron content and reduced number of viable oligodendrocytes in the periventricular area of the HI group compared with the NC group. No significant difference in iron content was observed between the HI + anemia and NC groups. The number of viable oligodendrocytes in the HI + anemia group was increased compared with that in the HI group, and the number in the HI + anemia group with late iron treatment was lower compared with that in the NC group and increased compared with that in the HI + anemia group. Electron microscopy revealed a significantly higher number of myelin sheaths in the HI + anemia group than in the HI group. IRP2 mRNA expression levels in the brain tissues were significantly decreased in the HI + anemia group compared with the HI group. The results suggest that anemia may reduce the rate of increase of iron content of the brain following HI. However, the early occurrence of anemia may protect against HIBI.