Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis
Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis
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流产布鲁氏菌粗突变体 RB51 疫苗接种可保护 BALB/c 小鼠免受流产布鲁氏菌、羊布鲁氏菌和绵羊布鲁氏菌的强毒菌株的侵害
DOI：
10.1128/iai.62.11.4990-4996.1994
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发表时间：
1994
影响因子：
3.1
通讯作者：
Winter
中科院分区：
文献类型：
--
作者：
P. M;Jimesnez;De;Bagus;T. P. H. Elzer;T. S. M. Jones;J. M. Blasco;F. Enright;G. Schurig;J. A.;Winter
用活流产布鲁氏菌 RB51（一种稳定的粗糙突变体）对 BALB/c 小鼠进行疫苗接种，可产生针对流产布鲁氏菌、羊布鲁氏菌和绵羊布鲁氏菌强毒株攻击的保护作用。被动转移实验表明，接种疫苗的小鼠通过细胞介导的免疫可免受流产布鲁氏菌 2308 的侵害，通过体液免疫可免受绵羊布鲁氏菌 PA 的侵害，通过两种形式的免疫可免受羊种布鲁氏菌 16M 的侵害。诱导保护性细胞介导的免疫需要活细菌；尽管产生了良好的迟发型超敏反应，但用 RB51 株的全灭活细胞进行疫苗接种未能保护小鼠免受流产布鲁氏菌 2308 的侵害。针对异源物种的保护性抗体主要是通过攻击感染后的记忆反应在接种疫苗的小鼠中产生的。抗原无关的单核细胞增生李斯特氏菌在接种疫苗的小鼠脾脏中的生长表明，残留的活化巨噬细胞的非特异性杀伤作用对保护作用微乎其微。这些结果鼓励继续研究 RB51 菌株作为针对异源布鲁氏菌的替代疫苗。然而，如本文所建议的，如果流产布鲁氏菌和绵羊布鲁氏菌之间不共享对保护性细胞介导的免疫至关重要的表位，则其针对绵羊布鲁氏菌的有用性将受到限制。
Vaccination of BALB/c mice with live Brucella abortus RB51, a stable rough mutant, produced protection against challenge with virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis. Passive-transfer experiments indicated that vaccinated mice were protected against B. abortus 2308 through cell-mediated immunity, against B. ovis PA through humoral immunity, and against B. melitensis 16M through both forms of immunity. Live bacteria were required for the induction of protective cell-mediated immunity; vaccination with whole killed cells of strain RB51 failed to protect mice against B. abortus 2308 despite development of good delayed-type hypersensitivity reactions. Protective antibodies against the heterologous species were generated in vaccinated mice primarily through anamnestic responses following challenge infections. Growth of the antigenically unrelated bacterium Listeria monocytogenes in the spleens of vaccinated mice indicated that nonspecific killing by residual activated macrophages contributed minimally to protection. These results encourage the continued investigation of strain RB51 as an alternative vaccine against heterologous Brucella species. However, its usefulness against B. ovis would be limited if, as suggested here, epitopes critical for protective cell-mediated immunity are not shared between B. abortus and B. ovis.
影响因子：
4.4
作者：
L. Araya;P. Elzer;G. Rowe;F. Enright;A. Winter
通讯作者：
L. Araya;P. Elzer;G. Rowe;F. Enright;A. Winter