Use of an immunomagnetic separation-fluorescent immunoassay (IMS-FIA) for rapid and high throughput analysis of environmental water samples
Use of an immunomagnetic separation-fluorescent immunoassay (IMS-FIA) for rapid and high throughput analysis of environmental water samples
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DOI：
10.1016/s0003-2670(98)00440-1
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发表时间：
1998-12-04
影响因子：
6.2
通讯作者：
Yu, H
中科院分区：
文献类型：
--
作者：
Yu, H
将基于抗体的免疫磁分离(IMS)与微孔板荧光免疫分析(FIA)相结合，用于环境水样中细菌和毒素的快速筛选。这种方法允许在分析时间的1小时内在同一微板上处理和分析多达96个样品。检测大肠埃希氏菌和葡萄球菌肠毒素B的灵敏度分别为2×10(3)细胞和10 ng/ml。这种独特的IMS-FIA方法也适用于餐饮原油样品的处理和分析，以及系统自动化。(C)1998年，爱思唯尔科学公司出版。保留所有权利。
An antibody-based immunomagnetic separation (IMS) in conjunction with microplate fluorescent immunoassay (FIA) was used for rapid screening of bacteria and toxin in environmental water samples. This approach allows processing and analysis of up to 96 samples on the same microplate within 1 h of assay time. The assay sensitivities are at the level of 2 x 10(3) cells for Escherichia coli and 10 ng/ml for Staphylococcal enterotoxin B detection, respectively. This unique IMS-FIA approach is also suitable for diner crude sample processing and analysis, as well as for system automation. (C) 1998 Published by Elsevier Science B.V. All rights reserved.