Differential effects of GDNF and BDNF on cultured ventral mesencephalic neurons
Differential effects of GDNF and BDNF on cultured ventral mesencephalic neurons
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DOI：
10.1016/s0169-328x(99)00015-7
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发表时间：
1999-03-20
期刊：
影响因子：
--
通讯作者：
Lu, B
中科院分区：
文献类型：
--
作者：
Feng, LY;Wang, CY;Lu, B
以往的研究表明，脑源性神经营养因子（BDNF）和胶质细胞源性神经营养因子（GDNF）可促进中脑腹侧多巴胺能神经元的存活。在这里，我们比较了几个非生存功能的两个因素在VM神经元的培养。我们发现BDNF和GDNF都能增加VM培养液中去极化诱导的多巴胺释放，但对GABA释放没有影响。BDNF而不是GDNF显著增强钙结合蛋白calbindin和突触蛋白SNAP 25的表达。相反，用GDNF而不是BDNF处理培养物，引起VM神经元突起的显著成束。因此，尽管BDNF和GDNF都作用于VM神经元，但它们具有不同的功能。(C)1999年由Elsevier Science B.V.出版，版权所有。
Previous studies have shown that brain derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) can enhance the survival of dopaminergic neurons in the ventral mesencephalon (VM). Here we compared several non-survival functions of the two factors in VM neurons in culture. We found that both BDNF and GDNF elicited an increase in the depolarization-induced release of dopamine, hut had no effect on GABA release, in the VM cultures. BDNF, but not GDNF, significantly enhanced the expression of the calcium binding protein calbindin and synaptic protein SNAP25, In contrast, treatment of the cultures with GDNF, but not BDNF, elicited a marked fasciculation of the processes of the VM neurons. Thus, although both act on VM neurons, BDNF and GDNF have distinct functions. (C) 1999 Published by Elsevier Science B.V. All rights reserved.