PLASMID TRANSFER BETWEEN MARINE-BACTERIA IN THE AQUEOUS PHASE AND BIOFILMS IN REACTOR MICROCOSMS
PLASMID TRANSFER BETWEEN MARINE-BACTERIA IN THE AQUEOUS PHASE AND BIOFILMS IN REACTOR MICROCOSMS
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DOI：
10.1128/aem.59.3.843-850.1993
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发表时间：
1993-03-01
影响因子：
4.4
通讯作者：
GOODMAN, AE
中科院分区：
文献类型：
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作者：
ANGLES, ML;MARSHALL, KC;GOODMAN, AE
在最佳条件下，通过使用质粒转移方法选择在接收和/或维持质粒方面比野生型更有效的SW 5受体，在营养板表面上将宽宿主范围质粒RP 1从海洋弧菌菌株S14转移到海洋菌株SW 5的质粒转移提高了2个数量级。该受体菌株SW 5 H用于在流动条件下在反应器中的玻璃珠表面上形成生物膜。在生物膜形成48或170小时后，将S142（RP 1）供体菌株引入反应器，并评估在没有选择压力的情况下在水相和生物膜中的transconjugants的产生。在170 h形成的生物膜中检测到质粒转移到生物膜中的受体细胞，但在48 h形成的生物膜中未检测到质粒转移。在生物膜中附着于珠表面的细胞中的质粒转移频率显著高于水相中的细胞中的质粒转移频率（P < 0.05）。
Plasmid transfer of broad-host-range plasmid RP1 from marine Vibrio sp. strain S14 to marine strain SW5 under optimum conditions on the surface of nutrient plates was improved 2 orders of magnitude by using the plasmid transfer process to select an SW5 recipient more efficient than the wild type in receiving and/or maintaining the plasmid. This recipient strain, SW5H, was used to form biofilms under flow conditions on the surfaces of glass beads in reactors. The S142(RP1) donor strain was introduced to the reactors after either 48 or 170 h of biofilm formation, and production of transconjugants in the aqueous phases and biofilms without selection pressure was assessed. Plasmid transfer to the recipient cells in the biofilm was detected for biofilms formed for 170 h but not in those formed for 48 h. The plasmid transfer frequency was significantly higher (P < 0.05) among cells attached to the bead surfaces in the biofilm than among cells in the aqueous phase.