Effect of bone marrow-derived CD11b+F4/80+ immature dendritic cells on the balance between pro-inflammatory and anti-inflammatory cytokines in DBA/1 mice with collagen-induced arthritis
Effect of bone marrow-derived CD11b+F4/80+ immature dendritic cells on the balance between pro-inflammatory and anti-inflammatory cytokines in DBA/1 mice with collagen-induced arthritis
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DOI：
10.1007/s00011-014-0707-7
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发表时间：
2014-05-01
影响因子：
6.7
通讯作者：
Wei, Wei
中科院分区：
文献类型：
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作者：
Fu, Jingjing;Zhang, Lingling;Wei, Wei
为探讨骨髓来源的CD11b(+)F4/80(+)未成熟树突状细胞(BM CD11b(+)F4/80(+)IDC)对胶原诱导性关节炎(CIA)DBA/1小鼠促炎和抗炎细胞因子平衡的影响。用rmGM-CSF和rmIL-4诱导BM CD11b(+)F4/80(+)IDC，并通过Toll样受体2(TLR-2)、吲哚乙胺2，3-脱氧酶(IDO)、白介素10(IL-10)、转化生长因子-β1和混合白细胞反应(MLR)。用II型胶原免疫DBA/1小鼠建立CIA模型。CIA小鼠免疫后3次静脉注射BM CD11b(+)F4/80(+)IDC。从关节炎指数、关节组织病理学、体重、胸腺指数、胸腺细胞增殖、IL-1β、肿瘤坏死因子α、IL-17、IL-10和转化生长因子-β1水平评价骨髓CD11b(+)F4/80(+)IDC对CIA的影响。经rmGM-CSF和rmIL-4诱导的BM CD11b(+)F4/80(+)IDC表达高水平的TLR-2、IDO、IL-10和TGF-β1。恢复体重，降低胸腺指数，抑制胸腺细胞增殖。BM CD11b(+)F4/80(+)IDC组小鼠IL-1β、TNF-α和IL-17水平降低，rmGM-CSF和rmIL-4可成功诱导BM CD11b(+)F4/80(+)IDC。BM CD11b(+)F4/80(+)IDC治疗可通过调节促炎细胞因子和抗炎细胞因子之间的平衡来改善CIA的发展和严重程度。
To explore the effect of bone marrow-derived CD11b(+)F4/80(+) immature dendritic cells (BM CD11b(+)F4/80(+)iDC) on the balance between pro-inflammatory and anti-inflammatory cytokines in DBA/1 mice with collagen-induced arthritis (CIA).BM CD11b(+)F4/80(+)iDC were induced with rmGM-CSF and rmIL-4, and were identified by the expressions of toll-like receptor 2 (TLR-2), indoleamine 2,3-deoxygenase (IDO), interleukin (IL)-10, transforming growth factor (TGF)-beta 1 and mixed leukocyte reaction (MLR). CIA was established in DBA/1 mice by immunization with type II collagen. CIA mice were injected intravenously with BM CD11b(+)F4/80(+)iDC three times after immunization. The effect of BM CD11b(+)F4/80(+)iDC on CIA was evaluated by the arthritis index, joint histopathology, body weight, thymus index, thymocytes proliferation, IL-1 beta, tumor necrosis factor (TNF)-alpha, IL-17, IL-10 and TGF-beta 1 levels.BM CD11b(+)F4/80(+)iDC induced with rmGM-CSF and rmIL-4 expressed high levels of TLR-2, IDO, IL-10 and TGF-beta 1. Infusion of BM CD11b(+)F4/80(+)iDC in CIA mice significantly reduced the arthritis index and pathological scores of joints, recovered the weight, decreased the thymus index and inhibited thymocyte proliferation. Levels of IL-1 beta, TNF-alpha and IL-17 were decreased in BM CD11b(+)F4/80(+)iDC-treated mice.BM CD11b(+)F4/80(+)iDC can be induced successfully with rmGM-CSF and rmIL-4. BM CD11b(+)F4/80(+)iDC treatment can ameliorate the development and severity of CIA by regulating the balance between pro-inflammatory cytokines and anti-inflammatory cytokines.