Effects of gossypol on rat Sertoli and Leydig cells in primary culture and established cell lines.
Effects of gossypol on rat Sertoli and Leydig cells in primary culture and established cell lines.
复制标题
棉酚对原代培养物和建立的细胞系中大鼠支持细胞和间质细胞的影响。
DOI：
10.1002/j.1939-4640.1983.tb02383.x
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发表时间：
1983
影响因子：
--
通讯作者：
Mather,JP
中科院分区：
文献类型：
--
作者：
Zhuang,LZ;Phillips,DM;Gunsalus,GL;Bardin,CW;Mather,JP
为了评价棉酚对睾丸细胞的直接作用，我们采用原代培养的大鼠睾丸间质细胞和支持细胞。在高达3μg/ml棉酚的培养3天中，间质细胞的存活、形态和睾酮的产生均未见改变。随着棉酚浓度的增加(3~7μ/ml)，细胞存活率下降，但雄激素分泌没有变化。相反，棉酚处理5天后，支持细胞的形态发生了显著的变化。在细胞质中出现了大的空泡和电子致密颗粒，但在从介质中去除棉酚的6天内，这些作用被逆转。在棉酚存在的情况下，支持细胞的雄激素结合蛋白(ABP)分泌显著减少。我们还测试了棉酚对三个已建立的细胞系生长的影响。两个Sertoli来源的细胞系TM4和TR-ST比Leydig来源的细胞系(TM3)更敏感。这些结果表明，在睾丸的体细胞类型中，支持细胞对棉酚最敏感。
In order to evaluate the direct effect of gossypol on testicular cells, we used primary cultures of rat Leydig and Sertoli cells. No alteration in Leydig cell survival, morphology, or testosterone production was seen during three days of culture with up to 3 μg/ml gossypol. With higher concentrations (3 to 7 (μ/ml) of gossypol, there was a reduction in cell survival but no change in androgen secretion. In contrast, there was a marked change in Sertoli cell morphology after five days of gossypol treatment. Large vacuoles and electron dense granules appeared in the cytoplasm, but these effects were reversed within six days of removing gossypol from the medium. There was a significant decrease in androgen binding protein (ABP) secretion by Sertoli cells in the presence of gossypol. We also tested the effect of gossypol on the growth of three established cell lines. Two Sertoli‐derived cell lines, TM4and TR‐ST, were more sensitive than a Leydig‐derived cell line (TM3). These results suggest that, of the somatic cell types in the testis, the Sertoli cells are most sensitive to gossypol.