PROPERTIES OF THE NAD(P)H-DEPENDENT XYLOSE REDUCTASE FROM THE XYLOSE-FERMENTING YEAST PICHIA-STIPITIS
PROPERTIES OF THE NAD(P)H-DEPENDENT XYLOSE REDUCTASE FROM THE XYLOSE-FERMENTING YEAST PICHIA-STIPITIS
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DOI：
10.1042/bj2260669
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
SCHEFFERS, WA
中科院分区：
文献类型：
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作者：
VERDUYN, C;VANKLEEF, R;SCHEFFERS, WA
来自木糖发酵酵母树干毕赤酵母的木糖还原酶通过离子交换、亲和和高性能凝胶色谱纯化，达到电泳和光谱均质性。该酶对多种醛糖底物具有活性，例如DL-甘油醛、L-阿拉伯糖、D-木糖、D-核糖、D-半乳糖和D-葡萄糖。因此，树干毕赤酵母的木糖还原酶是醛糖还原酶。与迄今为止表征的所有醛糖还原酶不同，来自该酵母的酶以 NADPH 和 NADH 作为辅酶具有活性。 NADH 的活性为.apprx。 70%的NADPH用于各种醛糖底物。 NADP 是 NADPH 和 NADH 连接的木糖还原的有效抑制剂，而 NAD 仅对 NADH 连接的反应表现出强烈的抑制作用。这些结果是在可能使用树干毕赤酵母和类似酵母将木糖厌氧转化为乙醇的背景下讨论的。
Xylose reductase from the xylose-fermenting yeast Pichia stipitis was purified to electrophoretic and spectral homogeneity via ion-exchanges, affinity and high-performance gel chromatography. The enzyme was active with various aldose substrates, such as DL-glyceraldehyde, L-arabinose, D-xylose, D-ribose, D-galactose and D-glucose. Hence the xylose reductase of Pichia stipitis is an aldose reductase. Unlike all aldose reductases characterized so far, the enzyme from this yeast was active with both NADPH and NADH as coenzyme. The activity with NADH was .apprx. 70% of that with NADPH for the various aldose substrates. NADP was a potent inhibitor of both the NADPH- and NADH-linked xylose reduction, whereas NAD showed strong inhibition only with the NADH-linked reaction. These results are discussed in the context of the possible use of Pichia stipitis and similar yeasts for the anaerobic conversion of xylose into ethanol.