Mechanisms regulating SHORT-ROOT intercellular movement
Mechanisms regulating SHORT-ROOT intercellular movement
复制标题
DOI：
10.1016/j.cub.2004.09.081
复制
发表时间：
2004-10-26
期刊：
影响因子：
9.2
通讯作者：
Benfey, PN
中科院分区：
文献类型：
--
作者：
Gallagher, KL;Paquette, AJ;Benfey, PN
发育中器官中的信号中心调节植物和动物的形态发生。短根转录因子(Short-Root，SHR)是一种组织信号，调节拟南芥根中特定干细胞的分裂。基因转录和蛋白质定位的比较表明，SHR以高度特异的方式从中柱细胞向外合成SHR。在这里，我们提供的证据表明，自发性高血压综合征细胞间贩运既受到监管，也有针对性。首先，我们证明了SHR在中柱中的亚细胞定位是SHR蛋白所固有的。接下来，我们证明SHR必须存在于细胞质中才能运动，这为SHR运动受到调控提供了证据。最后，我们描述了一种新的信息丰富的ShR等位基因，其中蛋白质存在于细胞质中，但不移动。因此，与通过类似扩散的过程移动的蛋白质相反，SHR的细胞质池不足以移动。
Signaling centers within developing organs regulate morphogenesis in both plants and animals. The putative transcription factor SHORT-ROOT (SHR) is an organizing signal regulating the division of specific stem cells in the Arabidopsis root. Comparison of gene transcription with protein localization indicates that SHR moves in a highly specific manner from the cells of the stele in which it is synthesized outward. Here, we provide evidence that SHR intercellular trafficking is both regulated and targeted. First, we show that subcellular localization of SHR in the stele is intrinsic to the SHR protein. Next, we show that SHR must be present in the cytoplasm to move, providing evidence that SHR movement is regulated. Finally, we describe an informative new shr allele, in which the protein is present in the cytoplasm yet does not move. Thus, in contrast to proteins that move by a process resembling diffusion, a cytoplasmic pool of SHR is not sufficient for movement.