Phylogenetic analysis of rotaviruses with genotypes G1, G2, G9 and G12 in Bangladesh: evidence for a close relationship between rotaviruses from children and adults
Phylogenetic analysis of rotaviruses with genotypes G1, G2, G9 and G12 in Bangladesh: evidence for a close relationship between rotaviruses from children and adults
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DOI：
10.1007/s00705-008-0212-9
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发表时间：
2008-11-01
影响因子：
2.7
通讯作者：
Naik, Trailokya Nath
中科院分区：
文献类型：
--
作者：
Paul, Shyamal Kumar;Kobayashi, Nobumichi;Naik, Trailokya Nath
为了阐明引起儿童和成人胃肠炎的轮状病毒的系统发育相关性，在2004年7月至2006年6月期间在孟加拉国的Mymensingh进行了流行病学调查。共分析了来自三家医院的2，540例腹泻患者的粪便标本。总体而言，儿童和成人轮状病毒阳性率分别为26.4%和10.1%。在155份来自儿童和成人的轮状病毒样本中，最常见的G基因型是G2（检出率分别为54.0%和47.6%），其次是G1（分别为21.2%和26.2%）和G9（分别为15.9%和9.5%）。G12还在5份标本中检出（共3.2%; 4名儿童和1名成人）。儿童和成人G2轮状病毒的VP 7基因序列同源性高于97.8%，而孟加拉国G2轮状病毒与世界其他地方报道的G2轮状病毒的同源性普遍较低，除了非洲国家报道的一些毒株。类似地，在儿童和成人之间观察到G1、G9和G12轮状病毒的VP 7基因以及P[4]、P[6]和P[8]病毒的VP 4基因具有极高的序列同一性。本研究中检测到的儿童和成人轮状病毒在单个G/P型的VP 7或VP 4基因的系统发育树图中被包括在单个簇中。轮状病毒有两个新出现的类型，G9和G12，其VP 7基因在遗传学上与最近在孟加拉国和印度报道的单个G型的基因接近，并且被包括在这些G型的全球传播谱系中。这些研究结果表明，在孟加拉国城市和农村地区，基因相同的轮状病毒，包括新出现的G9和G12型轮状病毒，正在儿童和成人中传播。
To clarify the phylogenetic relatedness of rotaviruses causing gastroenteritis in children and adults, an epidemiologic investigation was conducted in Mymensingh, Bangladesh, during the period between July 2004 and June 2006. A total of 2,540 stool specimens from diarrheal patients from three hospitals were analyzed. Overall, rotavirus-positive rates in children and adults were 26.4 and 10.1%, respectively. Among the 155 rotavirus specimens examined genetically from both children and adults, the most frequent G genotype was G2 (detection rate: 54.0 and 47.6%, respectively), followed by G1 (21.2 and 26.2%, respectively), and G9 (15.9 and 9.5%, respectively). G12 was also detected in five specimens (3.2% in total; four children and one adult). Sequence identities of VP7 genes of G2 rotaviruses from children and adults were higher than 97.8%, while these Bangladeshi G2 viruses showed generally lower identities to G2 rotaviruses reported elsewhere in the world, except for some strains reported in African countries. Similarly, extremely high sequence identities between children and adults were observed for VP7 genes of G1, G9 and G12 rotaviruses, and also for the VP4 genes of P[4], P[6], and P[8] viruses. Rotaviruses from children and adults detected in this study were included in a single cluster in phylogenetic dendrograms of VP7 or VP4 genes of individual G/P types. Rotaviruses with two emerging types, G9 and G12, had VP7 genes that were phylogenetically close to those of individual G-types recently reported in Bangladesh and India and were included in the globally spreading lineages of these G-types. These findings suggested that genetically identical rotaviruses, including those with the emerging types G9 and G12, were circulating among children and adults in city and rural areas of Bangladesh.