Separation of chromosomal length DNA molecules: pneumatic apparatus for rotating gels during electrophoresis.
Separation of chromosomal length DNA molecules: pneumatic apparatus for rotating gels during electrophoresis.
复制标题
染色体长度 DNA 分子的分离：电泳期间旋转凝胶的气动装置。
DOI：
10.1002/elps.1150100508
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Trunk,J
中科院分区：
文献类型：
--
作者：
Sutherland,JC;Emrick,AB;Trunk,J
通过周期性地改变电场相对于凝胶的方向超过90°，可以极大地增加凝胶电泳法可以分离的DNA分子的最大长度。一种方法是在电泳过程中周期性地将凝胶以所需的角度向交替方向旋转。我们描述了一种改进的旋转凝胶电泳仪，它使用气动旋转驱动器代替步进电机，从而降低了约50%的成本。我们设计的其他优点是重心更低，使设备更稳定，并从充满液体的电泳室下面移除所有电力。我们展示了从酿酒酵母334菌株中分离出的染色体长度的DNA分子在平行通道中被分离成14条可分辨的条带的数据。
The maximum length of DNA molecules that can be separated by gel electrophoresis can be increased greatly by periodically altering the direction of the electric field with respect to the gel by an angle that exceeds 90°. One method involves rotating the gel by the desired angle in alternate directions periodically during electrophoresis. We describe a modification of the rotating gel electrophoresis apparatus developed by Serwer (Electrophoresis1987, 8, 301–304) that uses a pneumatic rotary actuator instead of a stepping motor, hence reducing the cost by about 50 %. Other advantages of our design are a lower center of gravity that makes the apparatus more stable and the removal of all electrical power from beneath the fluid‐filled electrophoresis chamber. We present data demonstrating the separation of chromosomal length DNA molecules fromSaccharomyces cerevisiaestrain 334 into 14 resolved bands in parallel lanes.