TRANSMEMBRANE POTENTIAL AND IONIC CONTENT OF RAT ALVEOLAR MACROPHAGES
TRANSMEMBRANE POTENTIAL AND IONIC CONTENT OF RAT ALVEOLAR MACROPHAGES
复制标题
DOI：
10.1002/jcp.1041010313
复制
发表时间：
1979-01-01
影响因子：
5.6
通讯作者：
MILES, PR
中科院分区：
文献类型：
--
作者：
CASTRANOVA, V;BOWMAN, L;MILES, PR
测定大鼠肺泡巨噬细胞的细胞体积、细胞含水量、细胞内离子浓度和跨膜电位。对通过邻苯二甲酸二丁酯离心从培养基中分离的细胞进行测量，以大大减少捕获的细胞外空间。肺泡巨噬细胞的平均细胞体积为1525，其中72%是水。mu.ms细胞内液中Na+含量高（97 mM），K+含量低（50 mM），细胞内Cl-浓度为64 mM。通过使用荧光探针Di-S-C3（5）从氚化三苯基甲基鏻的平衡分布测量的跨膜电位约为-37 mV。Na+、K+和Cl-均不处于平衡状态。肺泡巨噬细胞膜的K+渗透性似乎大于Na+渗透性。
The cell volume, cell water, intracellular ionic concentrations and transmembrane potential of rat alveolar macrophages were determined. The measurements were made on cells which were separated from the medium by centrifugation through dibutyl phthalate to greatly reduce the trapped extracellular space. The mean cell volume of the alveolar macrophages is 1525 .mu.ms and 72% of this volume is water. The intracellular fluid is high in Na+ (97 mM) and lower in K+ (50 mM) and the intracellular Cl- concentration is 64 mM. The transmembrane potential, measured from the equilibrium distribution of tritiated triphenylmethyl phosphonium and by using the fluorescent probe, Di-S-C3(5), is approximately -37 mV. Neither Na+, K+ nor Cl- is distributed at equilibrium. The K+ permeability of alveolar macrophage membranes appears to be greater than Na+ permeability.