Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries
Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries
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DOI：
10.1111/j.1365-263x.2008.00942.x
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发表时间：
2009-03-01
影响因子：
3.8
通讯作者：
Kim, Young-Jae
中科院分区：
文献类型：
--
作者：
Choi, Eun-Jung;Lee, Sung-Hoon;Kim, Young-Jae
变形链球菌（Streptococcus mutans）和小链球菌（Streptococcus sobrinus）与幼儿龋齿（ECC）的发展密切相关。近年来，定量实时聚合酶链反应（qRT-PCR）被用于快速准确地定量这些细菌种类。本研究旨在通过qRT-PCR定量检测斑块样本中变形链球菌和sobrinus的水平，并评估它们与韩国学龄前儿童ECC患病率的关系。105例儿童（71月龄及以下）被分为3组（无龋、ECC、重度ECC）。收集牙菌斑样本，采用葡萄糖基转移酶基因特异性寡核苷酸引物（S. mutans-gtfB， S. sobrinus-gtfU）和通用引物进行qRT-PCR。采用Pearson相关检验来评估dmfs（龋坏、缺失或补牙表面）评分与微生物学结果之间的关系。三组牙菌斑标本中变形链球菌和sobrinus含量差异有统计学意义（P < 0.05）。sobrinus与S. mutans的比例与dmfs评分呈较强相关性（r = 0.748, P < 0.05）。本研究的qRT-PCR结果显示，ECC患儿牙菌斑样本中变形链球菌和sobrinus的含量较高。牙菌斑中sobrinus与S. mutans比例较高的患儿，其ECC发病率较高。
International Journal of Paediatric Dentistry 2008Streptococcus mutans and Streptococcus sobrinus are closely associated with the development of early childhood caries (ECC). Recently, quantitative real-time polymerase chain reaction (qRT-PCR) has been used for rapid and accurate quantification of these bacterial species.This study aims to detect quantitatively the levels of S. mutans and S. sobrinus in plaque samples by qRT-PCR, and to assess their association with the prevalence of ECC in Korean preschool children.One hundred and five children (71 months old or younger) were examined and classified into three groups (caries-free, ECC, severe ECC). Dental plaque samples were collected and qRT-PCR was conducted using oligonucleotide primers specific for glucosyltransferase gene (S. mutans-gtfB, S. sobrinus-gtfU) and universal primer. Pearson's correlation test was conducted to evaluate the relationship between the dmfs (decayed, missing, or filled surfaces primary teeth) scores and the microbiological findings.There was a significant difference between the levels of S. mutans and S. sobrinus in the plaque samples of the three groups (P < 0.05). The proportion of S. sobrinus to S. mutans showed strong correlation to the dmfs scores (r = 0.748, P < 0.05).The qRT-PCR results of this study showed that children with ECC had higher level of S. mutans and S. sobrinus in their dental plaque samples. The children with higher ratio of S. sobrinus to S. mutans in their dental plaque showed higher incidence of ECC.