Identification of the lectin-like receptor for oxidized low-density lipoprotein in human macrophages and its potential role as a scavenger receptor.
Identification of the lectin-like receptor for oxidized low-density lipoprotein in human macrophages and its potential role as a scavenger receptor.
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DOI：
10.1042/bj3340009
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发表时间：
1998-08
期刊：
影响因子：
--
通讯作者：
H. Yoshida;N. Kondratenko;S. Green;D. Steinberg;O. Quehenberger
中科院分区：
文献类型：
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作者：
H. Yoshida;N. Kondratenko;S. Green;D. Steinberg;O. Quehenberger
最近从牛内皮细胞和人肺中克隆了一种新的氧化型低密度脂蛋白受体--凝集素样氧化型低密度脂蛋白受体-1(LOX-1)。一项有限的组织分布研究表明，该蛋白主要由血管内皮细胞产生。在目前的研究中，我们证明LOX-1在巨噬细胞中也有表达，在巨噬细胞中它可能作为清道夫受体发挥作用。在未分化的THP-1细胞或新鲜分离的人单核细胞中未检测到LOX-1。然而，成熟的人单核细胞来源的巨噬细胞和分化的THP-1细胞显示出高水平的LOX-1转录本。免疫荧光染色和流式细胞仪分析证实LOX-1蛋白表达于巨噬细胞的质膜上。对巨噬细胞膜(但不是单核细胞膜)的Western-Blot分析发现了一条显示氧化低密度脂蛋白结合活性的单一条带，其表观分子质量约为40 kDa。这些结果表明，分化诱导巨噬细胞表达LOX-1，LOX-1可能作为清道夫受体和/或氧化低密度脂蛋白的受体发挥作用。
A new receptor for oxidized low-density lipoprotein (LDL), lectin-like oxidized LDL receptor-1 (LOX-1), has recently been cloned from bovine endothelial cells and human lung. A limited tissue-distribution study suggested that the protein was mainly produced by the vascular endothelium. In the present study we demonstrate that LOX-1 is also expressed in macrophages, where it may function as a scavenger receptor. LOX-1 was not detected in undifferentiated THP-1 cells or in freshly isolated human blood monocytes. However, mature human monocyte-derived macrophages and differentiated THP-1 cells showed high levels of LOX-1 transcripts. Consistent with these results, immunofluorescence staining and FACS analysis demonstrated that LOX-1 protein is expressed on the plasma membrane of macrophages. Western-blot analysis of membranes from macrophages (but not those from monocytes) identified a single band, with an apparent molecular mass of about 40 kDa, that displayed oxidized LDL-binding activity. These results suggest that differentiation induces the expression of LOX-1 in macrophages, where it may play a role as a scavenger receptor and/or a receptor for oxidized LDL.