Contractile skeletal muscle tissue-engineered on an acellular scaffold
Contractile skeletal muscle tissue-engineered on an acellular scaffold
复制标题
DOI：
10.1097/01.prs.0000101064.62289.2f
复制
发表时间：
2004-02-01
影响因子：
3.6
通讯作者：
Kuzon, WM
中科院分区：
文献类型：
--
作者：
Borschel, GH;Dennis, RG;Kuzon, WM
对于重建外科医生来说，组织工程化骨骼肌可以减少供体部位的发病率和无限的组织供应。作者使用去细胞小鼠趾长伸肌作为支架，制造了能够产生纵向力的工程化骨骼肌。使用作者实验室开发的方案，将来自成年小鼠的八块趾伸肌脱细胞。然后将无细胞肌肉置于含有20%胎牛血清的Dulbecco改良Eagle培养基和100 U/ml青霉素的浴中，室温下放置1周。使用26号针头和100-穆尔注射器将C2 C12成肌细胞注射到无细胞肌肉基质中。将所得构建体在37 ℃、5%二氧化碳下置于生长培养基中1周，每48小时更换培养基。然后将构建体置于分化培养基中1周，每48小时更换培养基。结构的等距收缩力测试证明了电刺激时产生纵向收缩力。观察到长度-张力或Starling关系。光学和电子显微镜研究表明，发育中的骨骼肌的一些正常组织学特征的重演。
For the reconstructive surgeon, tissue-engineered skeletal muscle may offer reduced donor-site morbidity and an unlimited supply of tissue. Using an acellularized mouse extensor digitorum longus muscle as a scaffold, the authors produced engineered skeletal muscle capable of generating longitudinal force. Eight extensor digitorum Ion-us muscles from adult mice were made acellular using a protocol developed in the authors' laboratory. The acellular muscles were then placed in a bath of 20% fetal bovine serum in Dulbecco's modified Eagle's medium and 100 U/ml penicillin for 1 week at room temperature. C2C12 myoblasts were injected into the acellular muscle matrix using a 26-gauge needle and a 100-mul syringe. The resulting constructs were placed in growth medium for 1 week at 37degreesC under 5% carbon dioxide, with media changes every 48 hours. The constructs were then placed in differentiation medium for I week, with media changes every 48 hours. Isometric contractile force testing of the constructs demonstrated production of longitudinal contractile force on electrical stimulation. A length-tension, or Starling, relationship was observed. Light and electron microscopy studies demonstrated recapitulation of some of the normal histologic features of developing skeletal muscle.