Effect of mammalian lignans on the growth of prostate cancer cell lines.
Effect of mammalian lignans on the growth of prostate cancer cell lines.
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发表时间：
2001-11
影响因子：
2
通讯作者：
Xuehua Lin;B. Switzer;W. Demark-Wahnefried
中科院分区：
文献类型：
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作者：
Xuehua Lin;B. Switzer;W. Demark-Wahnefried
背景技术哺乳动物木酚素、肠内酯(EL)和肠二醇(ED)已被证明可以抑制乳腺癌和结肠癌。迄今为止，尚未见木脂素对前列腺癌作用的报道。我们研究了 ED 和 EL 对三种人类前列腺癌细胞系（PC-3、DU-145 和 LNCaP）的影响。材料和方法用 0.1% (v/v) DMSO（载体）或 10-100 microM EL、ED 或染料木黄酮（阳性对照）处理细胞 72 小时。通过碘化丙啶核染色荧光测定法测量细胞活力，每次测定一式三份进行。结果在10-100μM时，EL显着抑制所有细胞系的生长，而ED仅抑制PC-3和LNCaP细胞。虽然 EL 是比 ED 更有效的生长抑制剂，但两者均不如金雀异黄酮有效。 EL 对 LNCaP 细胞生长抑制 50% 的剂量 (IC50) 为 57 µM，而 ED 的 IC50 为 100 µM（观察到的金雀异黄素 IC50 为 25 µM）。结论 ED 和 EL 抑制前列腺癌细胞的生长，并且可能通过激素依赖性和独立机制发挥作用。
BACKGROUND Mammalian lignans, enterolactone (EL) and enterodiol (ED), have been shown to inhibit breast and colon carcinoma. To date, there have been no reports of the effect of lignans on prostatic carcinoma. We investigated the effects of ED and EL on three human prostate cancer cell lines (PC-3, DU-145 and LNCaP). MATERIALS AND METHODS Cells were treated with either 0.1% (v/v) DMSO (vehicle) or 10-100 microM of EL, ED or genistein (positive control) for 72 hours. Cell viability was measured by the propidium iodide nuclei staining fluorometric assay with each assay performed in triplicate. RESULTS At 10-100 microM, EL significantly inhibited the growth of all cell lines, whereas ED only inhibited PC-3 and LNCaP cells. While EL was a more potent growth inhibitor than ED, both were less potent than genistein. The dose for 50% growth inhibition of LNCaP cells (IC50) by EL was 57 microM, whereas IC50 was 100 microM for ED, (the observed IC50 for genistein was 25 microM). CONCLUSION ED and EL suppress the growth of prostate cancer cells, and may do so via hormonally-dependent and independent mechanisms.