The endophytic fungi of Salvia miltiorrhiza Bge.f. alba are a potential source of natural antioxidants.
The endophytic fungi of Salvia miltiorrhiza Bge.f. alba are a potential source of natural antioxidants.
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DOI：
10.1186/s40529-015-0086-6
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发表时间：
2015-12
影响因子：
3.4
通讯作者：
Hao GP
中科院分区：
文献类型：
--
作者：
Li YL;Xin XM;Chang ZY;Shi RJ;Miao ZM;Ding J;Hao GP
白花丹参F.白花丹是一种传统中草药，对血栓闭塞性脉管炎有特殊的药理作用。然而，由于野生丹参的过度采集，其天然资源已严重不足。为了更好地利用这一资源，对分离的内生真菌的多样性和抗氧化活性进行了研究。丹参F.阿尔巴进行了调查。共分离到14株内生真菌。白花丹参通过形态学和分子生物学鉴定，将分离到的内生真菌分为4个属（Alternaria sp.，镰刀菌属，裂褶菌属和栓菌属）。这些真菌提取物使用乙醇制备和评价其植物化学化合物和抗氧化活性。链格孢菌SaF-2和增殖镰孢菌SaR-2特别令人感兴趣，因为它们产生所有9种植物化学物质，包括皂苷、酚、黄酮、强心苷、类固醇、单宁、生物碱、蒽醌和萜类化合物。F. proliferatum SaR-2和A. FRAP法和DPPH法测定表明，互隔交链孢霉SaF-2具有较强的抗氧化活性，其酚类和类黄酮含量高于植物根。酚类和类黄酮的总量分别为21.75、20.53没食子酸当量/g和8.27、7.36 μg/mg槲皮素当量。这两种内生真菌（SaR-2和SaF-2）对FRAP（1682.21和1659.05 μmol/mg）和DPPH自由基（90.14%和83.25%）的清除能力相当。这是首次报道分离到的内生真菌。白花丹参及其抗氧化活性这些结果表明，S.丹参芦荟可以是新型天然抗氧化剂的潜在来源。本文的在线版本（doi：10.1186/s40529-015-0086-6）包含补充材料，可供授权用户使用。
Salvia miltiorrhiza Bge. f. alba is a traditional Chinese herbal drug with special pharmacological effect on thromboangiitis obliterans. However, the nature source of S.miltiorrhiza Bge.f.alba is now in short supply because of the over-collection of the wild plant. To better utilize this resource, the diversity and antioxidant activity of endophytic fungi isolated from S. miltiorrhiza Bge. f. alba were investigated. A total of 14 endophytic fungi were isolated from different parts of S. miltiorrhiza Bge.f.alba. Based on morphological and molecular identification, the endophytic fungi isolated were classified into four genera (Alternaria sp., Fusarium sp., Schizophyllum sp. and Trametes sp.). These fungal extracts were prepared using ethanol and evaluated for their phytochemical compounds and antioxidant activity. Alternaria alternata SaF-2 and Fusarium proliferatum SaR-2 are of particular interest because they yielded all of nine phytochemicals including saponins, phenol, flavonoids, cardiac glycosides, steroids, tannins, alkaloids, anthroquinone and terpenoids. F. proliferatum SaR-2 and A. alternata SaF-2 also exhibited stronger antioxidant activities by FRAP and DPPH method, having the higher levels of phenol and flavonoid than those of plant root. The total amount of phenol and flavonoid quantified were of 21.75, 20.53 gallic acid equivalent per gram and 8.27 and 7.36 μg/mg of quercetin equivalent respectively. These two endophytic fungi (SaR-2 and SaF-2) were found to have comparable scavenging abilities on both FRAP (1682.21 and 1659.05 μmol/mg, respectively) and DPPH-free radicals (90.14% and 83.25%, respectively, at 0.1 mg/mL). This is the first report about isolation of endophytic fungi from S. miltiorrhiza Bge.f.alba and their antioxidant activities. These results indicate that the endophytic fungi associated with S. miltiorrhiza Bge.f. alba can be a potential source of novel natural antioxidants. The online version of this article (doi:10.1186/s40529-015-0086-6) contains supplementary material, which is available to authorized users.