Biomolecular effects of low-intensity ultrasound: Apoptosis, sonotransfection, and gene expression
Biomolecular effects of low-intensity ultrasound: Apoptosis, sonotransfection, and gene expression
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DOI：
10.1143/jjap.46.4435
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发表时间：
2007-07-01
期刊：
影响因子：
--
通讯作者：
Tachibana, Katsuro
中科院分区：
文献类型：
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作者：
Feril, Loreto B., Jr.;Kondo, Takashi;Tachibana, Katsuro
综述了低强度超声(US)在超声诱导的细胞程序性死亡(细胞凋亡)、基因表达调控以及超声介导的基因转染术(SonoThrating)等方面的生物学效应。研究表明，超声可以诱导细胞凋亡，并且在某些条件下可以提供最佳的细胞凋亡诱导。已有不同细胞系体外和体内靶向组织声学转染法的报道。几个基因也可以通过超声波上调或下调。就潜在的治疗应用而言，超声诱导细胞凋亡可能为肿瘤或癌组织的治疗提供直接、快速的途径。全身或局部声导入也可能是治疗局部和全身疾病的一种安全有效的基因治疗方法。靶细胞的基因调控可用于改变细胞对治疗的反应，例如增加患病细胞的敏感性，同时使正常细胞对治疗的副作用具有抵抗力。此外，US的基因调控也可能在促进受损组织的愈合方面发挥重要作用。
Biological effects of low-intensity ultrasound (US) focusing on US-induced programmed cell death (apoptosis), regulation of gene expression, and US-mediated gene transfection (sonotransfection) are reviewed. Studies have shown that US can induce apoptosis and that certain conditions can provide an optimal apoptosis induction. Sonotransfection of different cell lines in vitro and target tissues in vivo have been reported. Several genes can also be up-regulated or down-regulated by sonication. As to the potential therapeutic applications, apoptosis induction by US may induce direct and fast ways of treating tumor or cancer tissues. Systemic or local sonotransfection might also be a safe and effective gene therapy method in effecting the cure of local and systemic disorders. Gene regulation of target cells may be utilized in modifying cellular response to a treatment, such as increasing the sensitivity of diseased cells while making normal cells resistant to the side effects of the treatment. In addition, gene regulation by US may also play an important part in the enhanced healing of damaged tissues.