Alterations in the dynamics of inflammation, proliferation and apoptosis in subcutaneous implants of lupus-prone mice
Alterations in the dynamics of inflammation, proliferation and apoptosis in subcutaneous implants of lupus-prone mice
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DOI：
10.14670/hh-26.433
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发表时间：
2011-04-01
影响因子：
2
通讯作者：
Andrade, Silvia P.
中科院分区：
文献类型：
--
作者：
Campos, Paula P.;Vasconcelos, Anilton C.;Andrade, Silvia P.
伤口修复是一个复杂的过程，涉及炎症、增殖、细胞外基质沉积/重塑和细胞凋亡。自身免疫性疾病会严重影响愈合过程。我们使用组织学参数来描述在植入后10天和21天，易患狼疮的新西兰白鼠（NZW）和对照Balb/c小鼠品系皮下植入聚醚-聚氨酯海绵诱导的肥大细胞募集、增殖活性和纤维血管组织凋亡。实验期间，NZW小鼠种植体的纤维维管组织浸润（苏木精和伊红染色）、肥大细胞数量（Dominici染色）和细胞增殖（AgNOR染色）在实验早期（第10天）达到峰值，但胶原沉积（小天狼星红染色）和细胞凋亡仍然很高。相比之下，Balb/c动物植入物的肥大细胞募集和细胞增殖逐渐增加，但细胞凋亡从植入后10天到21天下降。在NZW小鼠中，早期肥大细胞募集、过多的胶原沉积和凋亡细胞从损伤部位移除的紊乱反应表明，NZW小鼠的基因型特征是异常愈合反应的决定因素。
Wound repair is a complex process that involves inflammation, proliferation, extracellular matrix deposition/remodeling and apoptosis. Autoimmune diseases profoundly affect the healing process. We have used histological parameters to characterize the recruitment of mast cells and the proliferative activity and apoptosis in the fibrovascular tissue induced by subcutaneous polyether-polyurethane sponge implants in lupus-prone New Zealand White (NZW) and in control Balb/c mouse strains at days 10 and 21 post implantation. Fibrovascular tissue infiltration (hematoxylin and eosin staining), mast cell number (Dominici staining) and cellular proliferation (AgNOR staining) peaked early (day 10) but collagen deposition (picrosirius red staining) and apoptosis remained high in implants of NZW mice during the experimental period. In contrast, implants of Balb/c animals showed a progressive increase in mast cell recruitment and cellular proliferation but apoptosis fell from day 10 to 21 post-implantation. This divergent response early mast cells recruitment, excessive collagen deposition and disturbed removal of apoptotic cells from the site of injury in NZW mice implies that the genotype trait of NZW mice is a determining factor in abnormal healing response.