Study on the expanded culture and kinetics of anammox bacteria in the upper flow packed bed.
Study on the expanded culture and kinetics of anammox bacteria in the upper flow packed bed.
复制标题
DOI：
10.1016/j.scitotenv.2018.08.404
复制
发表时间：
2019-02
期刊：
影响因子：
--
通讯作者：
Liang Qiao;Wen Xu;Shengxin Mao;Yan Li;Yanqiu Zhang
中科院分区：
文献类型：
--
作者：
Liang Qiao;Wen Xu;Shengxin Mao;Yan Li;Yanqiu Zhang
厌氧氨氧化（Anammox）技术在同时处理氨氮和亚硝酸盐氮方面具有独特的优势。动力学模型通常用于确定扩大的厌氧氨氧化反应器是否高效和稳定。高通量测序技术长期以来被用于厌氧氨氧化菌的鉴定。Monod模型表明，该工艺的理论最大总氮去除浓度接近1700 mgN/（gVSS. d）。Monod模型和Haltenet模型的动力学结果表明，亚硝酸盐氮对厌氧氨氧化菌有明显的抑制作用。Luong模型表明，进水总氮浓度仍有很大的改善潜力。修正的Stover-Kincannon模型和Grau二阶模型适用于描述反应器的稳定运行。高通量测序试验结果表明，该反应器厌氧氨氧化优势菌为革兰氏阴性杆菌，革兰氏阴性杆菌更适合该反应器的运行条件。有趣的是，在操作阶段，原始的细菌Anammoxoglobus逐渐被消除。反应器仍然具有相当高的去除底物的潜力。在扩大培养过程中，出现了菌种变异的现象，这在以往的文献中是比较少见的。
Anaerobic ammonium oxidation (Anammox) technology has a unique advantage in the simultaneous treatment of ammonia nitrogen and nitrite nitrogen. Kinetics models were usually utilized to identify an expanded anammox reactor to be efficient and stable. And the high-throughput sequencing test had been utilized to identify different kinds of anammox bacteria for a long time. The Monod model showed that the theoretical maximum total nitrogen removal concentration was near 1700 mgN/(gVSS·d). Nitrite nitrogen was an obvious inhibitor of anammox bacteria based on the kinetics results of both Monod model and Haldane model. The Luong model indicated that there was still a great potential of improvement of total influent nitrogen concentration. And the Modified Stover-Kincannon Model and Grau second-order model were applicable to describe stable operation of the reactor. While, high-throughput sequencing test results indicated that the bacteriaCandidatus Kueneniawas the dominant anammox bacteria of this reactor, which meant thatCandidatus Kueneniawas more applicable for operation condition of the reactor. Interestingly, the original bacteriumCandidatus Anammoxoglobuswas gradually eliminated during the operation phase. The reactor still had a quite high potential for the removal of the substrate. In the process of culture expansion, the phenomenon of bacterial species alteration had emerged, which was relatively rare in previous papers.