Simultaneous Detection of Pro- and Antioxidative Effects in the Variants of the Deoxyribose Degradation Assay
Simultaneous Detection of Pro- and Antioxidative Effects in the Variants of the Deoxyribose Degradation Assay
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DOI：
10.1021/jf902395k
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发表时间：
2010-02-24
影响因子：
6.1
通讯作者：
Chobot, Vladimir
中科院分区：
文献类型：
--
作者：
Chobot, Vladimir
对天然产物氧化还原特性的兴趣导致了各种检测抗氧化活性和清除活性氧特性的检测方法的发展。本文介绍了对2-脱氧-d -核糖降解试验的额外修改，特别允许测定测试化合物与抗坏血酸的自氧化和测试化合物本身的自氧化的相互作用。为了说明这一点，以核桃酮和槲皮素为例。改进的分析系统提供了深入了解其特定的抗氧化和促氧化性质。此外，如果铁离子以自由形式加入或与EDTA络合，则可以广泛表征其与铁配合物的氧化还原特性。与槲皮素-铁复合物相比，核桃素-铁复合物在更广泛的环境中被证明具有促氧化作用。
Interest in the redox properties of natural products has led to the development of various assays for the detection of antioxidant activities and ROS-scavenging properties. Here, additional modifications of the 2-deoxy-D-ribose degradation assay are introduced that specifically allow the determination of interactions of the test compound with the autoxidation of ascorbic acid and the autoxidation of the test compound itself. To illustrate this, juglone and quercetin were used as examples. The modified assay systems provide insights into their specific antioxidative and pro-oxidative properties. In additional, an extensive characterization of the redox properties of their complex with iron is possible, if iron ions are added in the free form or complexed with EDTA. The juglone-iron complex proved to be pro-oxidative in a wider range of milieus than the quercetin-iron complex.