Can membrane progesterone receptor α on T regulatory cells explain the ensuing human labour?
Can membrane progesterone receptor α on T regulatory cells explain the ensuing human labour?
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DOI：
10.1016/j.jri.2015.10.002
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发表时间：
2016-02-01
影响因子：
3.4
通讯作者：
Mota-Pinto, Anabela
中科院分区：
文献类型：
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作者：
Areia, Ana;Vale-Pereira, Sofia;Mota-Pinto, Anabela
黄体酮作为一种免疫类固醇，有助于建立妊娠保护环境。这似乎是黄体酮的责任，以避免导致分娩的炎症事件。T调节性淋巴细胞（Treg细胞）可以进一步解释黄体酮对这种细胞亚群的快速作用对导致分娩的炎症机制的抑制作用。我们研究了Treg细胞和这些免疫细胞中的膜孕酮受体α (mPR α)与人类分娩的关系。这项试点队列研究是在一个单中心三级产科单位进行的，有20名正常孕妇。通过流式细胞术计算CD4(+) T细胞、Treg细胞和mPR(α +) Treg细胞的绝对频率和相对频率的变化（妊娠中期和晚期；分娩当天）。我们的研究结果显示，在正常妊娠期间，从妊娠中期到妊娠晚期，Treg细胞和mPR(α +) Treg细胞普遍增加（分别为23.4%对52.3%和4.3%对8.3%）。相反，在分娩当天，与妊娠晚期相比，Treg细胞（52.3% vs. 17.4%）和mPR(α +) Treg细胞（8.3% vs. 6.1%）均突然下降。我们的研究结果表明，人类分娩可能是mPR(α +) Treg细胞下降的结果，mPR(α +) Treg细胞通过Treg细胞降低黄体酮的抗炎作用。2015爱思唯尔爱尔兰有限公司版权所有。
Progesterone acts as an immunosteroid by contributing to the establishment of a pregnancy-protective milieu. It seems that it is the responsibility of progesterone to evade the inflammatory events that lead to parturition. T regulatory lymphocytes (Treg cells) could further explain the inhibition of the inflammatory mechanisms that lead to labour through the rapid action of progesterone on this cell subset. We investigated Treg cells and the membrane progesterone receptor alpha (mPR alpha) in these immune cells with in relationship to human parturition. This pilot cohort study was conducted in a single-centre tertiary obstetrical unit with 20 normal pregnant women. Variation in the absolute and relative frequency of CD4(+) T cells, Treg cells, and of mPR(alpha+) Treg cells was calculated by flow cytometry on three occasions (second and third trimesters; delivery day). Our results show that during normal pregnancy there is a generalised increase in Treg cells and mPR(alpha+) Treg cells, from the second to the third trimesters (23.4% vs. 52.3% and 4.3% vs. 8.3%, respectively). On the contrary, on delivery day, compared with the values in the third trimester, there is a sudden decrease in both Treg cells (52.3% vs. 17.4%) and mPR(alpha+) Treg cells (8.3% vs. 6.1%). Our findings suggest that human labour may develop as a consequence of a decline in mPR(alpha+) Treg cells, which reduces progesterone anti-inflammatory action through Treg cells. (C) 2015 Elsevier Ireland Ltd. All rights reserved.