Effects of gonadotropin-releasing hormone agonists on meiotic maturation of follicle-enclosed oocytes in rabbits.
Effects of gonadotropin-releasing hormone agonists on meiotic maturation of follicle-enclosed oocytes in rabbits.
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DOI：
10.1095/biolreprod43.6.1012
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发表时间：
1990-12
影响因子：
3.6
通讯作者：
Yasunori Yoshimura;Yukio Nakamura;T. Oda;H. Yamada;T. Nanno;M. Ando;Y. Ubukata;M. Suzuki
中科院分区：
文献类型：
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作者：
Yasunori Yoshimura;Yukio Nakamura;T. Oda;H. Yamada;T. Nanno;M. Ando;Y. Ubukata;M. Suzuki
研究了GnRH激动剂对兔卵泡封闭卵母细胞体外成熟的影响。将兔排卵前卵泡在有或没有 hCG (10(2) ng/ml)、布舍瑞林 (10(2)-10(5) ng/ml) 或亮丙瑞林 (10(2)-10(5) ng/ml) 的情况下体外培养 14 小时。 GnRH 激动剂以剂量依赖性方式诱导卵泡封闭卵母细胞减数分裂的恢复。用 10(5) ng/ml 布舍瑞林 (87.9 +/- 6.3%) 或 10(5) ng/ml 亮丙瑞林 (86.0 +/- 4.1%) 治疗后达到 GVBD 的卵母细胞百分比与 hCG 治疗对照 (87.3 +/- 3.8%) 没有显着差异。成熟卵母细胞最初在接触 GnRH 激动剂后 2 小时内被检测到。同时添加 10(4) ng/ml 的 GnRH 拮抗剂可显着阻断 GnRH 激动剂对卵母细胞成熟的刺激作用。 GnRH 激动剂显着刺激排卵前卵泡产生前列腺素 (PG) E2 (PGE2) 和 PGF2 α（p 小于 0.01），但分泌的前列腺素水平在不同浓度的 GnRH 激动剂之间没有显着差异。暴露于 GnRH 激动剂后，卵泡封闭卵母细胞的减数分裂成熟开始时间比 PG 的产生早 2 小时。吲哚美辛显着减少 GnRH 激动剂刺激的 PG 产生。然而，GnRH 激动剂加吲哚美辛存在下的卵母细胞成熟度与单独使用 GnRH 激动剂的卵母细胞成熟度没有显着差异。 GnRH 激动类似物通过 PG 刺激以外的机制诱导兔子卵泡封闭卵母细胞减数分裂的恢复。
The effects of GnRH agonists on in vitro maturation of rabbit follicle-enclosed oocytes were studied. Rabbit preovulatory follicles were cultured with or without hCG (10(2) ng/ml), buserelin (10(2)-10(5) ng/ml), or leuprolide (10(2)-10(5) ng/ml) for 14 hours in vitro. GnRH agonists induced the resumption of meiosis in the follicle-enclosed oocytes in a dose-dependent manner. The percentage of oocytes achieving GVBD following treatment with 10(5) ng/ml buserelin (87.9 +/- 6.3%) or 10(5) ng/ml leuprolide (86.0 +/- 4.1%) did not differ significantly from hCG-treated control (87.3 +/- 3.8%). Mature oocytes initially were detected within 2 hours of GnRH agonist exposure. Concomitant addition of a GnRH antagonist at 10(4) ng/ml significantly blocked the stimulatory effect of GnRH agonist on oocyte maturation. GnRH agonists significantly stimulated both prostaglandin (PG) E2 (PGE2) and PGF2 alpha production by preovulatory follicles (p less than 0.01), but secreted prostanoid levels did not differ significantly among different concentrations of GnRH agonists. Meiotic maturation of follicle-enclosed oocytes following GnRH agonist exposure began 2 hours earlier than production of PGs. PG production stimulated by GnRH agonists was reduced significantly by indomethacin. However, oocyte maturity in the presence of GnRH agonist plus indomethacin did not differ significantly from that of GnRH agonist alone. GnRH agonistic analogues induce the resumption of meiosis in follicle-enclosed oocytes in rabbits by a mechanism other than PG stimulation.